anti-Amphiphysin, mAb (N8-2)

Product description: Monoclonal Antibody. Recognizes the Human, Mouse, Rat protein. Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol. Amphiphysins, members of the BAR (Bin-Amphiphysin-Rvsp) protein super family, play a key role in clathrin-mediated endocytosis of synaptic vesicles (SVs). All members of the BAR family share a highly conserved N-terminal BAR domain and a C-terminal Src homology (SH3) domain. Two isoforms of amphiphysin have been identified and act in concert as a heterodimer. Amphiphysins interact via its carboxy-terminal SH3 domain with dynamin, synaptojanin and clathrin. These complexes are implicated in synaptic vesicle recycling at nerve terminal.° Amphiphysins were also identified as the dominant autoantigen in paraneoplastic Stiff-Man Syndrome. May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton.

Alternate Names/Synonyms: AMPH; AMPH1; Amphiphysin

Product Type: Monoclonal Antibody

Clone: N8-2

Isotype: Mouse IgG1 kappa

Immunogen: Recombinant human GST-fragment protein (1-347AA) purified from E. coli.

Applications: ICC, IHC, IP, WB

Species Crossreactivity: Human, Mouse, Rat

Formulation: Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.

Short Term Storage: +4°C

Long Term Storage: -20°C

Shipping: BLUE ICE

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C.

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Literature References: 1) Zhang, B. and Zelhof, A.C. (2002) Traffic, 3:452-460. (General)2) Wigge, P. and McMahon, H.T. (1998) Trends Neurosci. 21, 339-344. (General)