anti-beta-Catenin (C-term), pAb

Product description: Polyclonal Antibody. Recognizes the Human, Mouse, Rat protein. Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol. beta-Catenin was a cytosolic protein originally identified through its association with the cadherin class of cell-adhesion proteins. beta-Catenin has two key cellular functions; one plays direct role in cell adhesion/migration, bridging between cadherins and tactin cytoskeleton. The other plays as a transcription cofactor with T cell factor/lymphoid enhancer factor (TCF/LEF) in the Wnt pathway. Wnt are powerful regulators of cell proliferation and differentiation, and their signaling pathway involves proteins that directly participate in both gene transcription and cell adhesion. Activation of Wnt signaling leads to inhibition of Glycogen synthase-3beta (GSK-3beta) activity, resulting in accumulation of cytoplasmic (signaling) beta-Catenin, which becomes available to bind the TCF/LEF family of transcription factors and to induce target gene expression. In the absence of Wnt signaling, A complex of axin and casein kinase-I(CK-I) induces beta-Catenin phospho- rylation at a single site: serine 45(Ser45). This likely serves as a priming site for subsequent phosphorylation by GSK3beta. And then beta-Catenin is phosphorylated by GSK-3beta at Ser33/37 and Thr41. These serine/threonine phosphylation is required for the phosph rylation-dependant degradation of beta catenin via ubiquitin-proteosome pathway. Mutation of these phosphorylation sites in beta-Catenin has been found in many tumor cell lines. Involved in the regulation of cell adhesion and in signal transduction through the Wnt pathway.

Alternate Names/Synonyms: CTNNB; CTNNB1; beta-Catenin; Catenin beta-1

Product Type: Polyclonal Antibody

Immunogen: Recombinant human His-beta-Catenin (C-terminal fragment) protein purified from E. coli.

Applications: IP, WB

Species Crossreactivity: Human, Mouse, Rat

Formulation: Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.

Short Term Storage: +4°C

Long Term Storage: -20°C

Shipping: BLUE ICE

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C.

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Literature References: 1) Hinck L, et al. (1994)Trends Biochem Sci. 19(12):538-42. (General)2) Schneider SQ, et al. (2003) J Exp Zoolog B Mol Dev Evol. 295(1):25-44. (General)3) W. James Nelson et al. (2004) Science 303 :1483-7. (General)4) Amit S, et al. (2002) Genes Dev. 16(9):1066-76. (General)(5) Morin P.J. et al. (1997) Science 275:1787-1790. (General)