anti-CAD (Caspase-activated Dnase), mAb (4B8)

Product description: Monoclonal Antibody. Recognizes the Human protein. Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol. Caspase-activated DNase (CAD) cleaves chromosomal DNA during apoptosis. Cancer which is a cell death process removing toxic and/or useless cells during mammalian development can be induced by a variety of stimuli, including the death factors of the tumor necrosis factor family, °or UV irradiation, anticancer drugs, and survival factor deprivation. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. CAD, which can be activated by caspases, exists as a complex with its inhibitor, inhibitor of CAD (ICAD). ICAD helps correct folding of CAD and remains complexed with CAD to inhibit the DNase activity of CAD. Caspase, which is activated during the apoptotic process, cleaves ICAD, and the CAD released from ICAD enters the nuclei to cleave the chromosomal DNA. Internal as well as external signals can activate caspases and this is under the control of the balance between pro-and anti-apoptotic proteins of the Bcl-2 family, heat shock proteins, and inhibitors of apoptosis proteins (IAPs). Currently, fourteen members of the caspase family have been identified, of which seven mediate apoptosis. Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.

Alternate Names/Synonyms: CAD; DFFB; CPAN; DFF-40; Caspase-activated DNase; Caspase-activated Nuclease; Caspase-activated Deoxyribonuclease; DNA Fragmentation Factor Subunit beta; DNA Fragmentation Factor 40 kDa Subunit

Product Type: Monoclonal Antibody

Clone: 4B8

Isotype: Mouse IgG2a kappa

Immunogen: Recombinant human His-CAD protein purified from E. coli.

Applications: ELISA, WB

Species Crossreactivity: Human

Formulation: Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.

Short Term Storage: +4°C

Long Term Storage: -20°C

Shipping: BLUE ICE

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C.

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Literature References: 1) Vermeulen K et al. (2005) Ann Hematol. 84(10):627-39. (General)2) Mukae N et al. (1998) Proc. Natl. Acad. Sci. U.S.A. 95:9123-9128. (General)3) Enari M et al. (1998) Nature 391:43-50. (General)