AVANTI POLAR LIPIDS , CYGNUS , LARODAN , ADIPOGEN , MYBIOSOURCE , SERVA NORDMARK, BIOWORLD, EDGEBIO , PANREAC APPLICHEM
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Product description: Monoclonal Antibody. Recognizes the Human protein. Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol. Human carboxypeptidase N (CPN) [(EC) 22.214.171.124], a member of the CPN/E subfamily of °regulatory° metallo-carboxypeptidases, is a zinc metalloprotease, which cleaves basic amino acids, lysine and arginine, from the carboxy-terminus of biologically active peptides and proteins. CPN is an extracellular glycoprotein synthesized in the liver and secreted into the blood, where it controls the activity of vasoactive peptide hormones, growth factors and cytokines by specifically removing C-terminal basic residues. Human CPN was discovered as an enzyme that inactivates bradykinin by cleaving its carboxy-terminal arginine and was also referred to as kininase I. CPN is a major inactivator of anaphylatoxins C3a, C4a and C5a and also cleaves off C-terminal Lys residues from larger protein substrates, such as the M and B subunits of creatine kinase, released from the heart after myocardial infarction. CPN is a tetramer (280°kDa) comprised of two heterodimers each consisting of a catalytic CPN1(48 ° 55 kDa) and non-catalytic CPN2 (83kDa) subunit.The CPN2 subunit keeps the smaller catalytic subunit in the circulation and protects it against inactivation at 37°°C. The CPN2 subunit may also promote the cleavage of higher molecular mass plasma protein substrates by the catalytic CPN1 subunit. The most conserved region of the carboxypeptidase proteins is the active site. All mammalian carboxypeptidases contain a zinc binding site, a substrate binding site, an amino acid involved in peptide specificity, and an amino acid involved in catalytic activity of the protein. Patients with reduced CPN level present chronic recurrent angioedema, which is unrelated to diet or environment. The 83 kDa subunit binds and stabilizes the catalytic subunit at 37 degrees Celsius and keeps it in circulation. Under some circumstances it may be an allosteric modifier of the catalytic subunit.
Alternate Names/Synonyms: CPN2; ACBP; Carboxypeptidase N Subunit 2; Carboxypeptidase N 83 kDa Chain; Carboxypeptidase N Large Subunit; Carboxypeptidase N Polypeptide 2; Carboxypeptidase N Regulatory Subunit
Product Type: Monoclonal Antibody
Isotype: Mouse IgG1 kappa
Immunogen: Protein purified from human plasma.
Applications: ELISA, WB
Species Crossreactivity: Human
Formulation: Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.
Short Term Storage: +4°C
Long Term Storage: -20°C
Shipping: BLUE ICE
Use & Stability: Stable for at least 1 year after receipt when stored at -20°C.
Literature References: 1. Keil C. et al. (2007) J Mol Biol. 2007 Feb 16;366(2):504-16. (General)2. Matthews KW, Mueller-Ortiz SL. and Wetsel RA. (2004) Mol Immunol. 40(11) :785-793. (General)
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