anti-Casein Kinase 1 delta, mAb (AF12G4)

Product description: Monoclonal Antibody. Recognizes the Human, Mouse, Rat protein. Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol. Casein kinases are a group of ubiquitous Ser/Thr kinases that phosphorylate key regulatory proteins involved in the control of cell differentiation, proliferation, chromosome segregation, circadian rhythms, and metabolic pathways. CK1 family members share a highly conserved kinase domain but differ in their variable N- and C-terminal domains. Mammals have seven family members: alpha, beta, gamma1, gamma2, gamma3, delta, and epsilon. They all share at least 50% amino acid identity within the protein kinase catalytic domain. CK1 isoforms exclusively use ATP as phosphate donor and are generally co-factor independent. Casein kinase 1 delta is ubiquitously expressed in all tissues, is p53 dependently induced in stress situations and plays an important role in various cellular processes. PKA phosphorylates CK1delta, predominantly at Ser370 in vitro and in vivo, and that site-specific phosphorylation of CK1delta by PKA plays an important role in modulating CK1delta-dependent processes. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. Retains PER1 in the cytoplasm.

Alternate Names/Synonyms: CKId; HCKID; CSNK1D; CKI-delta; EC=2.7.11.1; EC=2.7.11.26; Tau-Protein Kinase CSNK1D; Casein Kinase I Isoform delta

Product Type: Monoclonal Antibody

Clone: AF12G4

Isotype: Mouse IgG2b kappa

Immunogen: Recombinant human His-CK1d protein purified from E. coli.

Applications: ELISA, IP, WB

Species Crossreactivity: Human, Mouse, Rat

Formulation: Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.

Short Term Storage: +4°C

Long Term Storage: -20°C

Shipping: BLUE ICE

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C.

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Literature References: 1) Knippschild U et al. (2005) Cell Signal 17(6):675-89. (General)2) Stoter M et al, (2005) Oncogene 24(54):7964-75. (General)3) Giamas G et al, (2007) Biochem J 406(3):389-98. (General)