anti-Casein Kinase II alpha, mAb (27H4)

Product description: Monoclonal Antibody. Recognizes the Human, Mouse, Rat protein. Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol. The casein kinase I (CKI) family of serine/threonine protein kinases is highly conserved from yeast to humans. The CKI family is involved in many diverse and important cellular functions, such as regulation of membrane transport, cell division, DNA repair, circadian rhythms, and nuclear localization. The name of the enzyme family was originated from the convenience of casein as a substrate since the earliest days of research on protein phosphorylation. Casein kinase 2 (CK2) is a ubiquitous, highly conserved, essential serine/threonine kinase which has been implicated in cell cycle control, DNA repair, regulation of the circadian rhythm and other cellular processes. CK2 is a tetramer of two alpha subunits and two beta subunits. The alpha subunits have the catalytic kinase domain. Loss of the alpha° catalytic subunit produces male infertility, and loss of the single beta regulatory subunit is early embryonic lethal. CK2 appears to be upregulated in most cancers and the promotion of tumorigenesis by the overexpression of CK2 has been reported in transgenic mice. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha' chains contain the catalytic site. Participates in Wnt signaling. CK2 phosphorylates 'Ser-392' of p53/TP53 following UV irradiation.

Alternate Names/Synonyms: CK2A1; CSNK2A1; EC=2.7.11.1; CK II alpha; Casein Kinase II Subunit alpha

Product Type: Monoclonal Antibody

Clone: 27H4

Isotype: Mouse IgG2b kappa

Immunogen: Recombinant human His-Casein kinase II a protein purified from E. coli.

Applications: ELISA, WB

Species Crossreactivity: Human, Mouse, Rat

Formulation: Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.

Short Term Storage: +4°C

Long Term Storage: -20°C

Shipping: BLUE ICE

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C.

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Literature References: 1) Price M.A. 2006 Genes Dev. 20:399-410. (General)2) Seldin DC et al. (2005) Mol Cell Biochem. 274(1-2):63-67. (General)3) Tobin A.B. (2002) Trends Pharmacol Sci. 23:337-343. (General)4) Gross S.D. and Anderson R.A. (1998) Cell Signal. 10:699-711. (General)