anti-Cdc2, mAb (26E11)

Product description: Monoclonal Antibody. Recognizes the Human, Mouse, Rat protein. Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol. Cyclin-dependent kinases (cdk) belong to a group of protein kinases originally discovered as being involved in the regulation of the cell cycle. Cdks are also involved in the regulation of transcription and mRNA processing. A Cdk is activated by association with a cyclin, forming a cyclin-dependent kinase complex. Cdk1, also known as cell division control protein 2 (cdc2), is one of the components of the maturation promoting factor (MPF) which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Cdk1, when bound to cyclin B, allows a dividing cell to enter into mitosis from G2 and permits the transition from G1 through S in conjunction with cyclin A and cyclin E. The Cdk1 protein is constantly present throughout the cell division cycle, but its activity is finely tuned by means of protein-protein interactions and reversible phosphorylation. Cdk1 can also enhance cell migration. Increased levels of Cdk1 promote cell migration together with cyclin B2 and the actin-stabilizing protein caldesmon. Phosphorylation of caldesmon bound to actin results in the displacement of caldesmon from actin followed by the altered interaction of actin with myosin. These events contribute to increased cell migration.

Alternate Names/Synonyms: CDC2; CDK1; EC=2.7.11.22; EC=2.7.11.23; p34 Protein Kinase; Cyclin-dependent Kinase 1; Cell Division Protein Kinase 1; Cell Division Control Protein 2 Homolog

Product Type: Monoclonal Antibody

Clone: 26E11

Isotype: Mouse IgG2a kappa

Immunogen: Recombinant human His-cdc2 protein purified from E. coli.

Applications: ELISA, IP, WB

Species Crossreactivity: Human, Mouse, Rat

Formulation: Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.

Short Term Storage: +4°C

Long Term Storage: -20°C

Shipping: BLUE ICE

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C.

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Literature References: 1) Ferrari S, (2006) Cell Mol Life Sci. 63(7-8):781-795. (General)2) Stark GR and Taylor WR, (2004) Methods Mol Biol. 280:51-82. (General)3) Juliano R, (2003) Nat Cell Biol. 5(7):589-590. (General)