anti-cdk4, mAb (AF10A4)

Product description: Monoclonal Antibody. Recognizes the Human, Mouse, Rat protein. Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol. Cyclin-dependent kinases (Cdks) are the catalytic subunits of a family of mammalian heterodimeric serine/threonine kinases that have been implicated in the control of cell-cycle progression, transcription and neuronal function. Progression through G1 is regulated by a complex mechanism that can involve at least three Cdks (Cdk4, Cdk6 and Cdk2) and their regulators. Cyclins of the D-type family (D1, D2, and D3) bind and activate CDK4 and CDK6, while E-type cyclins (E1 and E2) primarily interact with CDK2. CDK4 and 6 are cyclin D1 binding partners, and activated cyclin D1/CDK4 and cyclin D1/CDK6 complex phosphorylate the retinoblastoma protein (Rb) to induce the expression of target genes essential for S phase entry, resulting in facilitation of the progression from G1 to S phase. Cdk4 and Cdk6 are inhibited by Cip/Kip or INK family of proteins. Probably involved in the control of the cell cycle.

Alternate Names/Synonyms: CDK4; PSK-J3; EC=2.7.11.22; Cyclin-dependent Kinase 4; Cell Division Protein Kinase 4

Product Type: Monoclonal Antibody

Clone: AF10A4

Isotype: Mouse IgG2a kappa

Immunogen: Recombinant human MBP-CDK4 (aa 1-303) protein purified from E. coli.

Applications: ELISA, WB

Species Crossreactivity: Human, Mouse, Rat

Formulation: Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.

Short Term Storage: +4°C

Long Term Storage: -20°C

Shipping: BLUE ICE

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C.

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Literature References: 1) Malumbres M and Barbacid M, (2005) Trends Biochem Sci 30(11) :630-41. (General)2) Fumi TY and Toshiyuki S, (2008) Cell Signal 20(4):581-9. (General)3) Lee YM and Sicinski P, (2006) Cell Cycle 5(18):2110-4. (General)