anti-Chk2 (Checkpoint Kinase 2), mAb (20A8)

Product description: Monoclonal Antibody. Recognizes the Human protein. Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol. Both Chk1 and Chk2 are critically important checkpoint kinases. Chk1 is essential for normal development and cell growth and Chk1 deficiency in mouse or in embryonic stem (ES) cells is lethal. Loss of Chk2 is tolerated and has been found to be associated with a subset of Li-Fraumeni cases. Li-Fraumeni syndrome increases greatly the susceptibility to cancer, with particularly high occurrences of breast cancer, brain tumors, acute leukemia, soft tissue sarcomas, bone sarcomas, and adrenal cortical carcinoma. Chk2 regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. The cell either stops the cell cycle and therefore its proliferation until the damage is repaired, or it destructs itself (apoptosis). The ataxia telangiectasia mutated (ATM) and ATR (ATM and Rad3-related) protein kinases exert cell cycle delay, in part, by phosphorylating Chk1, Chk2, and p53. Phosphorylation on Chk1 and p53 requires ATR, whereas phosphorylation on Chk2 requires ATM. Screening of novel substrates of Chk1 and Ckh2 followed by imunoprecipitation kinase assay and site-directed mutagenesis analysis suggests that HDAC5 and PGC-1 are specific targets for Chk1 and/or Chk2 at least in vitro. Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'.

Alternate Names/Synonyms: CDS1; hCds1; CHEK2; Hucds1; EC=2.7.11.1; Cds1 Homolog; Checkpoint Kinase 2; CHK2 Checkpoint Homolog; Serine/Threonine-Protein Kinase Chk2

Product Type: Monoclonal Antibody

Clone: 20A8

Isotype: Mouse IgG1 kappa

Immunogen: Recombinant human His-Chk2 protein purified from E. coli.

Applications: ELISA, IP, WB

Species Crossreactivity: Human

Formulation: Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.

Short Term Storage: +4°C

Long Term Storage: -20°C

Shipping: BLUE ICE

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C.

Lien vers la Fiche Technique

Literature References: 1) Kim MA et al. (2007) Exp Mol Med. 39(2):205-212. (General)2) Hammond EM et al. (2006) Cancer Lett. 238(2):161-167. (General)3) Helt CE et al. (2005) J Biol Chem. 280(2):1186-1192. (General)