anti-Erk1, mAb (33A5)

Product description: Monoclonal Antibody. Recognizes the Human, Rat protein. Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol. ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and post-mitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase, and activates it as well. Once actived, Raf phosphorylates other two kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in ERK 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1. Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1. Phosphorylates heat shock factor protein 4 (HSF4).

Alternate Names/Synonyms: ERT2; ERK1; ERK-1; MAPK3; MAPK 3; p44-ERK1; p44-MAPK; EC=2.7.11.24; MAP Kinase 3; MAP Kinase Isoform p44; Insulin-stimulated MAP2 Kinase; Mitogen-activated Protein Kinase 3

Product Type: Monoclonal Antibody

Clone: 33A5

Isotype: Mouse IgG2a kappa

Immunogen: Recombinant human His-Erk1 protein purified from E. coli.

Applications: ELISA, WB

Species Crossreactivity: Human, Rat

Formulation: Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.

Short Term Storage: +4°C

Long Term Storage: -20°C

Shipping: BLUE ICE

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C.

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Literature References: 1) Smalley, K. (2003) Int. J. Cancer, 104, 527-532. (General)2) Johnson, G.L. and Lapadat, R. (2002) Science, 298, 1911-1912. (General)3) Kolch, W. (2000) Biochem. J. 351, 289-305. (General)