GAPDH (human) (rec.)

Product Description: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a catalytic enzyme commonly known to be involved in glycolysis. The enzyme exists as a tetramer of identical 37-kDa subunits. GAPDH catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehydes 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, this ubiquitously expressed enzyme also displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling (1-5). Other studies also provide evidence of GAPDH playing an essential part of the program of gene expression observed in apoptosis and as part of the cellular phenotype of age-related neuro-degenerative diseases (6-7).

Alternate Names/Synonyms: GAPD; GAPDH; EC=2.6.99.-; EC=1.2.1.12; Peptidyl-Cysteine S-Nitrosylase GAPDH; Glyceraldehyde-3-Phosphate Dehydrogenase

Product type: Protein

Source / Host: E. coli

Species Crossreactivity: Yeast

SBiological Activity: 25.6U/mg (One unit will cause the oxidation of 1.0 µmole of NADPH per minute by DeltaA412/0.0136×2)

Purity: >95% (SDS-PAGE)

Formulation: Lyophilized in 20mM HEPES, pH 7.4.

Handling Advice: Avoid freeze/thaw cycles.

Short Term Storage: +4°C

Long Term Storage: -20°C

Shipping: BLUE ICE

Use & Stability: After reconstitution, store at -80°C.

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