anti-HA Tag, mAb (LF7H5)

Product description: Monoclonal Antibody. Recognizes the All protein. Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol. Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage. The expression vectors are used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. Short pieces of well defined peptides (Poly-His, Flag-epitope or cmyc epitope or HA-tag) or small proteins (bacterial GST, MBP) is often cloned along with the target gene. The HA tag is derived from an epitope of the influenza hemagglutinin protein, which has been extensively used as a general epitope tag in expression vectors. It has been successfully used in a variety of applications, including Western blotting, Immunoprecipitation, and immunofluorescence studies.

Alternate Names/Synonyms: HA Epitope Tag

Product Type: Monoclonal Antibody

Clone: LF7H5

Isotype: Mouse IgG1 kappa

Immunogen: Recombinant human HA-tag protein purified E. coli.

Applications: ELISA, ICC, IP, WB

Species Crossreactivity: All

Formulation: Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50%glycerol.

Short Term Storage: +4°C

Long Term Storage: -20°C

Shipping: BLUE ICE

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C.

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Literature References: 1) K. Terpe. (2003) Appl Microbiol Biotechnol 60: 523-533. (General)2) Maiattis T. et. al. 1982 Molecular Cloning. (General)3) Hopp T.P. et. al. 1988. Biotechnology 6:1204-1210. (General)4) Smith D.B. 1988. Gene 67:31-40. (General)