anti-HePTP, mAb (AFPK32)

Product description: Monoclonal Antibody. Recognizes the Human protein. Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol. Hematopoietic tyrosine phosphatase (HePTP, PTPN7) belongs to a subgroup of PTPases with two other members, STEP and PCPTP1. HePTP is a 38°kDa class I non-receptor protein tyrosine phosphatase (PTP) that is strongly expressed in T cells. The protein consists of a short, amino-terminal extension that contains a conserved 16-amino acid kinase interaction motif (KIM) and a carboxy-terminal protein tyrosine phosphatase domain. HePTP binds the MAPK family kinases, Erk1/2 and p38 via KIM. Erk phosphorylates HePTP (at Thr45 and Ser72) and HePTP dephosphorylates Erk (at Tyr185). HePTP is phosphorylated at Ser23 within the KIM by PKA in T cells. This phosphorylation results in dissociation of the HePTP/ERK2 complex and reduced ability of HePTP to dephosphorylate ERK. Protein phosphatase that acts preferentially on tyrosine-phosphorylated MAPK1. Plays a role in the regulation of T and B-lymphocyte development and signal transduction.

Alternate Names/Synonyms: HEPTP; PTPN7; EC=3.1.3.48; Protein-Tyrosine Phosphatase LC-PTP; Hematopoietic Protein-Tyrosine Phosphatase; Tyrosine-Protein Phosphatase Non-Receptor Type 7

Product Type: Monoclonal Antibody

Clone: AFPK32

Isotype: Mouse IgG1 kappa

Immunogen: Recombinant human protein purified from E. coli.

Applications: ELISA, WB

Species Crossreactivity: Human

Formulation: Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.

Short Term Storage: +4°C

Long Term Storage: -20°C

Shipping: BLUE ICE

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C.

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Literature References: 1) Mustelin T et al. (2005) J Mol Biol 354:150-63. (General)2) Eswaran J et al. (2006) Biochem J 395:483–491. (General)3) Nika K et al. (2004) Biochem J 378:335-342. (General)