anti-ICAD, mAb (AF41E3)

Product description: Monoclonal Antibody. Recognizes the Human protein. Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol. The inhibitor of caspase-3-activated DNase (ICAD) is a caspase 3 substrate that controls nuclear apoptosis. ICAD has two isoforms: a functional isoform of 45kDa, ICAD-L/DNA fragmentation factor (DFF) 45; and a 35kDa isoform, ICAD-S/DFF35. Although both ICAD-L and ICAD-S can bind and inhibit CAD, only ICAD-L was reported to be functional. ICAD is cleaved to be inactivated and allow caspase-activated DNase (CAD) to execute nuclear internucleosomal apoptotic DNA fragmentation. In non-apoptotic cells, CAD is complexed with its inhibitor, ICAD. The activation of the CAD/ICAD complex occurs through the caspase 3-mediated cleavage of ICAD at residues 117 and 224, which results in three ICAD fragments that are then released from CAD. In addition to its DNase inhibitory activity, ICAD acts as a CAD specific folding chaperone. There are recent reports that ICAD is a potential target for restoring a normal apoptotic signal transduction pathway in colon and brain cancer cells. Inhibitor of the caspase-activated DNase (DFF40).

Alternate Names/Synonyms: H13; DFF1; ICAD; DFFA; DFF-45; Inhibitor of CAD; DNA Fragmentation Factor Subunit alpha; DNA Fragmentation Factor 45 kDa Subunit

Product Type: Monoclonal Antibody

Clone: AF41E3

Isotype: Mouse IgG2a kappa

Immunogen: Recombinant human His-ICAD protein purified from E. coli.

Applications: ELISA, IP

Species Crossreactivity: Human

Formulation: Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.

Short Term Storage: +4°C

Long Term Storage: -20°C

Shipping: BLUE ICE

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C.

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Literature References: 1) Enari M et al, Nature. (1998) vol.391(6662): pp.43-50. (General)2) Sakahira H et al, J Biol Chem. (1999) vol.274(22): p.15740-4. (General)3) Charrier L et al, Cancer Res. (2002) vol.62(7): p.2169-74. (General)4) Fukushima K et al, J Mol Biol. (2002) vol.321(2): p.317-27. (General)