anti-IKK-alpha (3A11)

Product description: Monoclonal Antibody. Recognizes the Human, Mouse, Rat protein. Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol. IkappaB kinase alpha (IKKalpha) is a component of a multiprotein kinase complex that regulates the activity of the transcription factor NF-kappaB. Activation of the IKK complex via upstream stimuli leads to phosphorylation and degradation of NF-kappaB bound IkappaB(Inhibitor of kappaB). Subsequently, free NF-kappaB dimers enter the nucleus and regulate the transcription of a variety of target genes involved in cell proliferation and differentiation, apoptosis, and inflammation, etc. The IKK complex consists of 2 highly homologous kinase subunits, IKKalpha and IKKbeta, and a nonenzymatic regulatory component, IKKgamma/NEMO. The relative contributions of IKKalpha and IKKbeta to the signalling complex vary according to the requirements of the cell. IKKbeta plays a predominant role in immune responses, while IKKalpha alone appears to be sufficient for at least some developmental systems. Recently, IKK/NF-kappaB signaling pathway was studied as therapeutic targets in cancer. Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines.

Alternate Names/Synonyms: IKKA; CHUK; IKK1; IkBKA; IKK-A; TCF-16; NFKBIKA; IKK-alpha; EC=2.7.11.10; IkappaB Kinase; I-kappa-B Kinase 1; I-kappa-B Kinase alpha; Transcription Factor 16

Product Type: Monoclonal Antibody

Clone: 3A11

Isotype: Mouse IgG1 kappa

Immunogen: Synthetic peptide.

Applications: IP, WB

Species Crossreactivity: Human, Mouse, Rat

Formulation: Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.

Short Term Storage: +4°C

Long Term Storage: -20°C

Shipping: BLUE ICE

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C.

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Literature References: 1) Luo JL et al, J Clin Invest. (2005) vol.115(10): pp.2625-32. (General)2) DiDonato JA. Sci STKE. (2001) vol.2001(97): pp.PE1. (General)3) Clarkson R. Breast Cancer Res. (2002) vol.4(5): pp.173–5. (General)4) Kim HJ, et al, Cell Death Differ. (2006) vol.13(5): pp.738-47. (General)