anti-MEK2, mAb (1A1)

Product description: Monoclonal Antibody. Recognizes the Human protein. Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol. The MAP Kinase pathway is a key signaling mechanism that regulates many cellular functions such as cell growth, proliferation, differentiation, development, transformation and apoptosis. The basic arrangement includes a G-protein, a MAPK kinase kinase (MAPKKK) that phosphorylates and activates a MAPK kinase (MAPKK), which in turn phosphorylates MAPK. The ERK (extracellular-signal regulated kinase) cascade is a central MAPK pathway which contains Ras as a G protein, Raf as a MAPKKK, MEK1 (MAPK/ERK kinase1) and MEK2 (MAPK/ERK kinase2) as MAPKK, and ERKs as MAPK. MEK1 and MEK2 are °80% identical to each other, and are essentially identical in most of their kinase domain. MEK1 and MEK2 phosphorylate ERKs equally well, both in vivo and in vitro. MEK1 (45 KDa) and MEK2 (46 KDa) are composed of a catalytic kinase domain, which is surrounded by a regulatory N-terminal domain (°80 amino acids) and a shorter C-terminal region (°30 amino acids). Unlike the kinase domains, the N-termini and the characteristic Pro-rich inserts are quite divergent between the two MEKs (40% identity). MEKs are activated by phosphorylation of two Ser residues in their activation loop (Ser218 and Ser222 in MEK1) located within a Ser°Xaa°Ala°Xaa°Ser/Thr motif, typical to all MAPKKs. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases

Alternate Names/Synonyms: MEK2; MEK 2; MAP2K2; MAPKK 2; EC=2.7.12.2; MAPK/ERK Kinase 2; MAP Kinase Kinase 2; ERK Activator Kinase 2; Dual Specificity Mitogen-activated Protein Kinase Kinase 2

Product Type: Monoclonal Antibody

Clone: 1A1

Isotype: Mouse IgG1 kappa

Immunogen: Recombinant human His-MEK2 protein purified from E. coli.

Applications: IP, WB

Species Crossreactivity: Human

Formulation: Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.

Short Term Storage: +4°C

Long Term Storage: -20°C

Shipping: BLUE ICE

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C.

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Literature References: 1) Shaul Y.D. and Seger R. (2007) Biochim Biophys Acta. 1773:1213-1226. (General)2) Ravingerova T. et al. (2003) Mol Cell Biochem. 247:127-138. (General)3) Kolch W. (2000) Biochem J. 351 Pt2:289-305. (General)