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Ref. ALG-PN10811-KI01
Alithea Genomics

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Neuf 1850.00 1665.00 H.T.

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Détails Produit

Alithea's MERCURIUS BRB-seq™ Library Preparation Kit is ideal for 3’ mRNA Sequencing of Bulk RNA samples, which means large-scale RNA sequencing (transcriptomic) studies such as biomarker discovery, antibody development and drug screening. It enables cost-effective, high-throughput RNA-seq library preparation from a broad range of purified RNA or cell lysate RNA (i.e. no RNA extraction required) samples. Bulk RNA Barcoding followed by sequencing (BRB-seq™) is Alithea's flagship technology. It is based on early barcoding followed by pooling. With BRB-seq™, hundreds of RNA samples can be processed in a single tube immediately after the first reverse transcription reaction. This leads to a significant reduction in reagents and manual effort in the entire workflow. The MERCURIUS BRB-seq™ kits offer an optimized set of barcodes that ensure consistent and reproducible data generation from a large number of samples. In addition to applications in human medicine, BRB-seq™ can also be readily applied to model organisms, plants and in vitro models. This product is useful for drug discovery, RNA sequencing (Transcriptomics) and other research areas and suited for research laboratories in academia or industry (pharma, biotech or agricultural) who are interested in analyzing many samples at once.

Also available is the MERCURIUS Blood BRB-seq Library Preparation Kit (#ALG-PN10821) suitable for blood RNA samples (i.e. globin depletion is built into the BRB-seq™ workflow).

Benefits of MERCURIUS

Fast: 10x fewer manual steps / High Throughput
Cost-efficient: 10x less reagent consumption / Low Cost
Robust: Reduced technical variability by processing 96 samples as one
Accurate: PCR duplicates are removed by using unique molecular identifiers (UMI)
All-in-One: Globin depletion is integrated in the workflow at no extra steps (ALG-PN10821)

 

Product Details
Synonyms Barcoded RNA-Sequencing Kit
Product Type Kit
Properties
Application Set RNA Sequencing
Crossreactivity All
Quantity

Contains sufficient materials for 96 samples

Sample Type Purified RNA
Cell Lysate RNA
Kit Contains

Provided as 2 module boxes: 

Barcoded Oligo-dT Adapters Module (PN 10511)
Library Preparation and UDI Module (PN 10521)

Please see the Manual for an overview of all components included in this kit. See Techincal Note for additional information on the included inline control RNA sample.

Shipping and Handling
Shipping DRY ICE
Short Term Storage -20°C
Long Term Storage -20°C
Handling Advice Avoid freeze/thaw cycles.
Use/Stability 12 months after the day of manufacturing. See expiry date on Kit box.
Documents
Manual Download PDF
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

 

Product References
  1. BRB-seq: ultra-affordable high-throughput transcriptomics enabled by bulk RNA barcoding and sequencing: D. Alpern, et al.; Genome Biol. 20, 71 (2019)
  2. Systems-Genetics-Based Inference of a Core Regulatory Network Underlying White Fat Browning: Y. Li, et al.; Cell Rep. 29, 4099 (2019)
  3. Sex-dependent and sex-independent regulatory systems of size variation in natural populations: H. Okada, et al.; Mol. Syst. Biol. 15, e9012 (2019)
  4. Commensal Gut Bacteria Buffer the Impact of Host Genetic Variants on Drosophila Developmental Traits under Nutritional Stress: D. Ma, et al.; iScience 19, 436 (2019)
  5. cis-regulatory variation modulates susceptibility to enteric infection in the Drosophila genetic reference panel: M.V. Frochaux, et al.; Genome Biol. 21, 6 (2020)
  6. Extensive tissue-specific expression variation and novel regulators underlying circadian behavior: M. Litovchenko et al.; Sci. Adv. 7, eabc3781 (2021)
  7. Disparate temperature-dependent virus–host dynamics for SARS-CoV-2 and SARS-CoV in the human respiratory epithelium: P. V'kovski, et al.; PLoS Biol 19, e3001158 (2021)
  8. The Hippo pathway controls myofibril assembly and muscle fiber growth by regulating sarcomeric gene expression: A. Kaya-Copur, et al.; eLife 10, e63726 (2021)