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100 µl

Ref. YIF-LF-MA0372

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Neuf 331.00 H.T.


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Détails Produit

Product description: Monoclonal Antibody. Recognizes the Human protein. Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol. Dual specificity protein phosphatase 1 (also known as HVH1; MKP1; CL100; MKP-1; PTPN10)is an enzyme that in humans is encoded by the DUSP1 gene [1, 2]. The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation [3]. DUSP1 has been shown to interact with MAPK14 [4][5], MAPK1 [5][6] and MAPK8 [5]. Dual specificity phosphatase that dephosphorylates MAP kinase MAPK1/ERK2 on both 'Thr-183' and 'Tyr-185', regulating its activity during the meiotic cell cycle.

Alternate Names/Synonyms: CL100; DUSP1; MKP-1; EC=; EC=; MAP Kinase Phosphatase 1; Protein-Tyrosine Phosphatase CL100; Dual Specificity Protein Phosphatase 1; Dual Specificity Protein Phosphatase hVH1

Product Type: Monoclonal Antibody

Clone: PK4-1B8

Isotype: Mouse IgG2b

Immunogen: Recombinant human protein purified from E. coli.

Applications: IP, WB

Species Crossreactivity: Human

Formulation: Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.

Short Term Storage: +4°C

Long Term Storage: -20°C

Shipping: BLUE ICE

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C.

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Literature References: 1) Keyse SM and Emslie EA (1992), "Oxidative stress and heat shock induce a human gene encoding a protein-tyrosine phosphatase". Nature 359 (6396): 644–7. (General)2) Martell KJ, et al. (1995), "Chromosomal localization of four human VH1-like protein-tyrosine phosphatases". Genomics 22 (2): 462–4. (General)3) "Entrez Gene: DUSP1 dual specificity phosphatase 1" (General)4) Tanoue, T, et al. (2001), "A Novel MAPK phosphatase MKP-7 acts preferentially on JNK/SAPK and p38 alpha and beta MAPKs". J. Biol. Chem. (United States) 276 (28): 26629–39. (General)5) Slack, DN, et al. (2001), "Distinct binding determinants for ERK2/p38alpha and JNK map kinases mediate catalytic activation and substrate selectivity of map kinase phosphatase-1". J. Biol. Chem. (United States) 276 (19): 16491–500. (General)6) Calvisi, DF, et al. (2008), "Dual-specificity phosphatase 1 ubiquitination in extracellular signal-regulated kinase-mediated control of growth in human hepatocellular carcinoma". Cancer Res. (United States) 68 (11): 4192–200. (General)