AVANTI POLAR LIPIDS , CYGNUS , LARODAN , ADIPOGEN , MYBIOSOURCE , SERVA NORDMARK, BIOWORLD, EDGEBIO , PANREAC APPLICHEM
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Product description: Monoclonal Antibody. Recognizes the Human, Mouse, Rat protein. Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol. Mitogen-activated protein kinases (MAPKs) are activated by various extracellular signals, such as growth factors, stresses, and cytokines, and play crucial roles in the determination of cell fate through proliferation, differentiation, survival, and apoptosis. Mnk1(MAP kinase-interacting serine/threonine-protein kinase 1) and Mnk2 are protein kinases that are directly phosphorylated and activated by extracellular signal-regulated kinase (ERK) or p38 mitogen-activated protein (MAP) kinases which phosphorylate at least two threonine residues (Thr197 and Thr202 of the mouse Mnk1) located in the activation loop. Mnks are implicated in the regulation of protein synthesis through their phosphorylation of eukaryotic translation initiation factor 4E (eIF4E), also known as cap-binding protein. The initiation step of translation is rate-limiting and represents a major target for translational control. The formation of the cap-binding complex eIF4F, which is a multiprotein factor responsible for the recruitment of the 40S ribosomal subunit to the mRNA 5? end, is a key regulatory step. Mnk1 is physically associated with eIF4F and directly phosphorylate eIF4E, a component of the eIF4F complex. eIF4E specifically binds the 5? m7GpppN cap structure found in all eukaryotic mRNAs and plays a critical role in cap-dependent translation initiation as a central component of the eIF4F complex, which also contains eIF4A and eIF4G. May play a role in the response to environmental stress and cytokines. Appears to regulate transcription by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.
Alternate Names/Synonyms: Mnk1; MNK1; MKNK1; EC=220.127.116.11; MAPK Signal-integrating Kinase 1; MAP Kinase Signal-integrating Kinase 1; MAP Kinase-interacting Serine/Threonine-Protein Kinase 1
Product Type: Monoclonal Antibody
Isotype: Mouse IgG1 kappa
Immunogen: Recombinant human His-Mnk1 (aa 1-362) protein purified from E. coli.
Applications: ELISA, WB
Species Crossreactivity: Human, Mouse, Rat
Formulation: Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.
Short Term Storage: +4°C
Long Term Storage: -20°C
Shipping: BLUE ICE
Use & Stability: Stable for at least 1 year after receipt when stored at -20°C.
Literature References: 1) Ueda T et al. (2004) Mol Cell Biol. 24(15):6539-6549. (General)2) Pyronnet S, (2000) Biochem Pharmacol. 60(8):1237-1243. (General)3) Fukunaga R and Hunter T, (1997) EMBO J. 16(8):1921-1933. (General)
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