AVANTI POLAR LIPIDS , CYGNUS , LARODAN , ADIPOGEN , MYBIOSOURCE , SERVA NORDMARK, BIOWORLD, EDGEBIO , PANREAC APPLICHEM
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Product description: Polyclonal Antibody. Recognizes the Human protein. Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol. Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. The nucleosome in which an 147 base-pairs of DNA are wrapped 1.7 times around a central core of eight histone protein molecules forms the basic building block of chromatin. Histone octamer consists of two copies each of H2A, H2B, H3, and H4 histones. Histone H2A variant H2AX, a component of the nucleosome core structure that comprises 10%°15% of total cellular H2A, has a special role in DNA repair, cell cycle checkpoints, regulated gene recombination events, and tumor suppression. H2AX can be phosphorylated on Ser 1, acetylated on Lys 5 and ubiquitinated on Lys 119. But what makes H2AX unique is a highly conserved serine 139 residue which is rapidly phosphorylated upon the exposure of cells to DNA damage. H2AX is phosphorylated by ATR in response to DNA replication stress, and primarily by ATM in response to low levels of ionizing radiation. Phosphorylation of H2AX increases the likelihood of assembling a functional repair complex by increasing the local concentration of repair factors near the lesion. Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
Alternate Names/Synonyms: H2AX; H2AFX; H2a/x; Histone H2AX; Histone H2A.X
Product Type: Polyclonal Antibody
Immunogen: Synthetic peptide.
Species Crossreactivity: Human
Formulation: Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.
Short Term Storage: +4°C
Long Term Storage: -20°C
Shipping: BLUE ICE
Use & Stability: Stable for at least 1 year after receipt when stored at -20°C.
Literature References: 1) Stucki M, et al. (2005), Cell. 123(7):1213-1226. (General)2) Fernandez-Capetillo O, et al. (2004), DNA Repair (Amst). 3(8-9):959-967. (General)3) Emmy P. Rogakou, et al. (1998), J Biol Chem. 273(10):5858-5868. (General)
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