anti-PIWIL2, mAb (11C11)

Product description: Monoclonal Antibody. Recognizes the Mouse protein. Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol. The piwi gene of Drosophila belongs to a novel class of evolutionarily conserved genes (the piwi or Argonaute family). The Piwi family° is required for germ- and stem-cell development in invertebrates, and two Piwi members, MIWI and MILI (Miwi like; Piwil2; Piwi-like protein 2), are essential for spermatogenesis in mouse. MIWI and MILI partner with Piwi-interacting RNAs (piRNAs) which are 26°31 nucleotides (nt) in length°clearly distinct from the 21°23°nt of µRNAs (miRNAs) or short interfering RNAs (siRNAs). A search for murine small RNAs that might program Piwi proteins for transposon suppression revealed developmentally regulated piRNA loci, some of which resemble transposon master control loci of Drosophila. MILI might be programmed by distinct piRNA populations at different stages of germ cell development, suggesting MILI plays an important role in meiotic prophase progression. Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells. Its presence in oocytes suggests that it may participate to similar functions during oogenesis in females. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with primary piRNAs in the cytoplasm and is required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. The piRNA process acts upstream of known mediators of DNA methylation. Participates to a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Indirectly modulate expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8. Inhibits tumor cell growth when repressed. When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors.

Alternate Names/Synonyms: CT80; HILI; PIWIL2; Piwi-like Protein 2; Cancer/Testis Antigen 80

Product Type: Monoclonal Antibody

Clone: 11C11

Isotype: Mouse IgG1 kappa

Immunogen: Recombinant human GST-mili-1 protein purified from E. coli.

Applications: ELISA, IP, WB

Species Crossreactivity: Mouse

Formulation: Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.

Short Term Storage: +4°C

Long Term Storage: -20°C

Shipping: BLUE ICE

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C.

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Literature References: 1) Aravin AA et al. (2007) Science. 316(5825):744-747. (General)2) Aravin A et al. (2006) Nature. 442(7099):203-207. (General)3) Kuramochi-Miyagawa S et al. (2004) Development. 131(4):839-849. (General)