anti-PP2A-alpha, pAb

Product description: Polyclonal Antibody. Recognizes the Human, Mouse, Rat protein. Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol. Protein Phosphatase 2A (PP2A) is one of the major Ser/Thr phosphatases implicated in the regulation of many cellular processes including regulation of different signal transduction pathways, cell cycle progression, DNA replication, gene transcription and protein translation. The core structure comprises a 36 kDa catalytic subunit (PP2AC) and a 65 kDa regulatory subunit (PR65 or A subunit). Each PP2A subunit has at least two isoforms and the catalytic subunit, present in the alpha and beta isoforms, share 97% homology. The differential association of all these subunits gives rise to an extensive subset of oligomeric holoenzymes. It is widely thought that PP2A exercises regulatory flexibility and differential substrate specificity through the specific association of the core dimer (PP2AD) with one of the three regulatory B subunits. Moreover, PP2A interacts with a still growing number of cellular and viral proteins and is regulated by posttranslational modifications. PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Can dephosphorylate SV40 large T antigen and p53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen.

Alternate Names/Synonyms: RP-C; PPP2CA; PP2A-alpha; EC=3.1.3.16; Replication Protein C; Serine/Threonine-Protein Phosphatase 2A Catalytic Subunit alpha Isoform

Product Type: Polyclonal Antibody

Immunogen: Synthetic peptide.

Applications: WB

Species Crossreactivity: Human, Mouse, Rat

Formulation: Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.

Short Term Storage: +4°C

Long Term Storage: -20°C

Shipping: BLUE ICE

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C.

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Literature References: 1) Janssens V. et al, (2005) Curr Opin Genet Dev. vol.15(1): pp.34-41. (General)2) Garcia A. et al, (2003) Biochimie. vol.85(8): pp.721-6. (General)3) Van Hoof C. et al, (2004) Cancer Cell. vol.5(2): pp.105-6. (General)4) Van Hoof C. et al, (2003) Biochim Biophys Acta. vol.1640(2-3): pp.97-104. (General)5) Lechward K. et al, (2001) Acta Biochim Pol. vol.48(4): pp.921-33. (General)