anti-PRAK, mAb (13H5)

Product description: Monoclonal Antibody. Recognizes the Human, Mouse, Rat protein. Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol. PRAK is a 471 amino acid protein with 20-30% sequence identity to the known MAP kinase-regulated protein kinases RSK1/2/3, MNK1/2 and MAPKAPK2/3. The p38 mitogen-activated protein kinase (MAPK) pathway plays an important role in cellular responses to inflammatory stimuli and environmental stress. There are at least six protein kinases that can be regulated by p38alpha and/or p38beta. These downstream kinases of p38s include MAPK-activated protein kinase 2 (MAPKAPK2 or MK2), MAPKAPK3, MAPK-interacting kinase 1 (MNK1), MNK2, p38-activated/regulated protein kinase (PRAK or MAPKAPK5), and mitogen- and stress-activated protein kinase (MSK). PRAK can be activated in response to cellular stress and proinflammatory cytokines. T182 within the activation loop of PRAK has been determined to be the regulatory phosphorylation site. PRAK has been reoprted to be essential for ras-induced senescence and tumor suppression. PRAK mediates senescence upon activation by p38 in response to oncogenic ras. Mediates stress-induced small heat shock protein 27 phosphorylation.

Alternate Names/Synonyms: MK5; MK-5; PRAK; MAPKAPK5; MAPKAP-K5; MAPKAPK-5; EC=2.7.11.1; MAPKAP Kinase 5; MAPK-activated Protein Kinase 5; MAP Kinase-activated Protein Kinase 5; p38-regulated/activated Protein Kinase

Product Type: Monoclonal Antibody

Clone: 13H5

Isotype: Mouse IgG1 kappa

Immunogen: Recombinant human His-PRAK protein purified from E. coli.

Applications: ELISA, WB

Species Crossreactivity: Human, Mouse, Rat

Formulation: Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.

Short Term Storage: +4°C

Long Term Storage: -20°C

Shipping: BLUE ICE

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C.

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Literature References: 1) Sun P. et al. (2007) Cell. 128:295-308. (General)2) New L. et al. (1998) EMBO J. 17:3372-3384. (General)