anti-SHP2, mAb (5F2)

Product description: Monoclonal Antibody. Recognizes the Human, Mouse, Rat protein. Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol. SHP2 is a tyrosine phosphatase containing two tandem SH2 (src homology 2) domains. Protein tyrosine phosphatases (PTPs) are a group of enzymes that remove phosphate groups from phosphorylated tyrosine residues on proteins. Together with tyrosine kinases, PTPs regulate the phosphorylation state of many important signaling molecules. SHP1 and SHP2 represent a subfamily of non-transmembrane PTPs that contain two SH2 domains followed by a PTP domain. Tyrosine phosphorylation of SHP2 is required for normal ERK activation in response to some growth factors. In the inactive state, the N-terminal SH2 domain binds the PTP domain and blocks access of potential substrates to the active site. This auto-inhibition is relieved by the binding of phosphopeptides to SH2 domain, resulting in activation of phostphatase activity. Noonan syndrome characterized by an abnormal face, short stature and cardiac abnormalities is due to the mutations of N-terminal SH2 domain or PTP domain. SHP2 mutations might also cause some leukemia, and could be important in pathogenesis by Helicobacter pylori, the major cause of gastric ulcer and carcinoma. Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus.

Alternate Names/Synonyms: Shp2; PTP2C; SHP-2; PTP-2C; PTP-1D; PTPN11; SH-PTP3; SH-PTP2; EC=3.1.3.48; Protein-Tyrosine Phosphatase 1D; Protein-Tyrosine Phosphatase 2C; Tyrosine-Protein Phosphatase Non-Receptor Type 11

Product Type: Monoclonal Antibody

Clone: 5F2

Isotype: Mouse IgG1 kappa

Immunogen: Recombinant human GST-SHP2 protein purified from E. coli.

Applications: IP, WB

Species Crossreactivity: Human, Mouse, Rat

Formulation: Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.

Short Term Storage: +4°C

Long Term Storage: -20°C

Shipping: BLUE ICE

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C.

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Literature References: 1) Salmond R.J. and Alexander D.R. (2006) Trends Immunol. 27:154-160. (General)2) Neel B.G. et al. (2003) Trends Biochem Sci. 28:284-293. (General)3) Agazie M. and Hayman M.J. (2003) Mol Cell Biol. 23:7875-7886. (General)4) Araki T. et al. (2003) J Biol Chem. 278:41677-41684. (General)5) Yu D.H. et al. (1998) J Biol Chem. 273:21125-21131. (General)