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100 µl

Ref. YIF-LF-MA0077
AB FRONTIER

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Neuf 321.00 H.T.

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Détails Produit

Product description: Monoclonal Antibody. Recognizes the Human protein. Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol. Thioredoxins (Trx) are small, multi-functional proteins with oxidoreductase activity and are ubiquitous in essentially all living cells. Trx contains a redox-active disulfide/ dithiol group within the conserved Cys-Gly-Pro-Cys active site. The two cysteine residues in the conserved active centers can be oxidized to form intramolecular disulfide bonds. Reduction of the active site disulfide in oxidized Trx is catalyzed by Trx reductase with NADPH as the electron donor. The reduced Trx is a hydrogen donor for ribonucleotide reductase, the essential enzyme for DNA synthesis, and a potent general protein disulfide reductase with numerous functions in growth and redox regulations. Specific protein disulfide targets for reduction by Trx include protein disulfide°isomerase (PDI) and a number of transcription factors such as p53, NF-kappaB and AP-1 (T1-151). Trx is also capable of removing H2O2, particularly when it is coupled with either methionine sulfoxide reductase or several isoforms of peroxiredoxins (6-7). Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).

Alternate Names/Synonyms: TXN; ADF; Trx; TRDX; SASP; Thioredoxin; ATL-derived Factor; Surface-associated Sulphydryl Protein

Product Type: Monoclonal Antibody

Clone: 8A1

Isotype: Mouse IgG1 kappa

Immunogen: Recombinant human protein purified from E. coli.

Applications: ELISA, IP

Species Crossreactivity: Human

Formulation: Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.

Short Term Storage: +4°C

Long Term Storage: -20°C

Shipping: BLUE ICE

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C.

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Literature References: 1) Andoh, T. et al. (2002) J.Biol.Chem. 277, 9655-9660. (General)2) Arner, E. S. and Holmgren, A. (2000) Eur. J. Biochem. 267, 6102-6109. (General)3) Lundstrom, J. and Holmgren, A. (1990) J. Biol. Chem. 265, 1994-9120. (General)4) Nordberg, J. and Arner, E. S. J. (2001) Free Radic. Biol. Med. 31, 1287-1312. (General)5) Matthews, J. R. et al. (1992) Nucleic Acids Res. 20, 3821-3830. (General)6) Wei, S. J. (2000) Cancer Res. 60, 6688-6695. (General)7) Chae, H. Z. (1999) Methods Enzymol. 300, 219-226. (General)