anti-Vimentin, mAb (33C7)

Product description: Monoclonal Antibody. Recognizes the Human, Rat protein. Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol. Vimentin is a member of the intermediate filament family of proteins found in various non-epithelial cells, especially mesenchymal cells. Vimentin is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. Vimentin plays a significant role in supporting and anchoring the position of the organelles in the cytosol. Although most intermediate filaments are stable structures, vimentin also has a dynamic nature which is important when offering flexibility to the cell. Two monomers which have central alpha-helical domains, capped on each end by non-helical domains twist around each other to form a coiled-coil dimer. Two dimers then form a tetramer, which, in turn, form a sheet by interacting with other tetramers. There are some reports related to the biochemical function of intermediate filament network. The intracellular movement of LDL-derived cholesterol from the lysosome to the site of esterification is a vimentin-dependent process. A role for vimentin in mechanotransduction of shear stress has also been suggested. The mechanical stress of fluid shear on endothelial cells seems to trigger MAPK signaling pathways and stimulates proliferation. Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.

Alternate Names/Synonyms: VIM; Vimentin

Product Type: Monoclonal Antibody

Clone: 33C7

Isotype: Mouse IgG1 kappa

Immunogen: Recombinant human His-Vimentin protein purified from E. coli.

Applications: ELISA, IP, WB

Species Crossreactivity: Human, Rat

Formulation: Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.

Short Term Storage: +4°C

Long Term Storage: -20°C

Shipping: BLUE ICE

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C.

Lien vers la Fiche Technique

Literature References: 1) Pekny M and Lane EB, (2007) Exp Cell Res. 313(10):2244-2254 (General)2) Holwell TA et al. (1999) J Lipid Res. 40(8):1440-1452. (General)3) Henrion D et al. (1997) J. Clin. Invest. 100:2909–2914 (General)4) Sarria AJ et al. (1992) J Biol Chem. 267(27):19455-19463. (General)