2-Iminobiotin Separopore® (Agarose) 6B-CL

Applications:

2-Iminobiotin-Separopore 6B-CL is used for the purification of avidin/streptavidin conjugates. Proteins coupled to (strept)avidin are more readily recovered in native form from 2-Iminobiotin-Separopore than the biotin resin. It is also used for removal of free avidin from (strept)avidin conjugate preparations. Interaction with avidin and streptavidin is pH dependent: forms stable complex above pH 9.5 and dissociates at pH 4.

Technical specifications:

• Ligand: 2-Iminobiotin
• Matrix: Separopore 6B-CL (crosslinked agarose beads, 6%)
• Particle size range: 52 - 165 µm
• Matrix activation: Amino
• Molecular weight range: 6 x 10⁴ - 2 x 10⁷
• Binding capacity: 4 mg avidin / ml drained gel
• pH stability: 4 - 9
• Flow specifications: 70 - 140 cm / h
• Supplied as suspension in 0.5M NaCl, 0.01M sodium phosphate (pH 6.8) and 0.02% sodium azide
• Appearance: White to off-white gel suspension

References:

• Complexes of streptavidin-fused antigens with biotinylated antibodies targeting receptors on dendritic cell surface: a novel tool for induction of specific T-cell immune responses. Mol Biotechnol. (2012) 51: 221-32.
• Efficient production of active chicken avidin using a bacterial signal peptide in Escherichia coli. Biochem J. (2004) 384: 385-90.
• A new europium beta-diketone chelate for ultrasensitive time-resolved fluorescence immunoassays. Anal Biochem. (2002) 311: 57-67.
• Production and purification of two recombinant proteins from transgenic corn. Biotechnol Prog. (1998) 14: 149-55.

Related products:

• Biotin-Separopore 6B-CL (SKU # 20181061)

Usage : bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.