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1 Kit

Ref. SBB-KP0038-KI01
SOUTH BAY BIO

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Neuf 389.00 H.T.
en stock

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Détails Produit

Product description: SOUTH BAY BIO's kit is designed to test for specific activity of 20S proteasome. The kit provides purified 20S proteasome and is designed to test for Chymotrypsin-like activity (Suc-LLVY-AMC), Caspase-like activity of the proteasome subunits beta1/PSMB6 (LLE-AMC) and Chymotrypsin-like activity of beta5/PSMB5 (WLA-AMC). Additionally, the compound MG-132 is included, which can be used to inhibit the proteasome. All peptide substrates are conjugated to AMC, which upon proteasome catalyzed hydrolyses display fluorescence at Excitation=345nm, Emission=445nm; allowing for a real-time read out of 20S proteasome specific activity.

Product Type: Kit

Specificity: Readout: Endpoint/Kinetic; Label or Dye: 7-Amino-4-methylcoumarin; Detection Method: Fluorescence; Substrate Properties: Protein-based Substrate.; Excitation/Emission (nm): 345/445

Crossreactivity: Human

Kit Contains: 10x 20S Proteasome; 50x LLVY-AMC, Chemotrypsin-like activity; 50x WLA-AMC, beta5/PSMB5 specific substrate; 50x LLE-AMC, beta1/PSMB6 specific substrate; 50x Inhibitor (MG-132, 2mM) in 100% DMSO; 10x Reaction Buffer; 50x SDS(1.75%) in H20; 100x free AMC Standard (40µM)

Short Term Storage: -20°C

Long Term Storage: -80°C

Shipping: DRY ICE

Handling Advice Aliquot to avoid freeze/thaw cycles. Avoid freeze/thaw cycles.

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