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1 Kit

Ref. SBB-KP0037-KI01
SOUTH BAY BIO

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Neuf 495.00 H.T.
en stock

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Détails Produit

Product description: SOUTH BAY BIO's kit is designed to test for specific activity of 20S immunoproteasome. The kit provides purified 20S immunoproteasome and is designed to test for Chymotrypsin-like activity (Suc-LLVY-AMC) and Caspase-like activity of the immunoproteasome subunits beta1i/ PSMB9 (PAL-AMC) and beta5i/PSMB8 (ANW-AMC). Additionally, the compound, ONX-0914, which can be used to inhibit specifically the subunit beta5i/LMP7 20S immunoproteasome is included. All peptide substrates are conjugated to AMC, which upon proteasome catalyzed hydrolyses display fluorescence at Excitation=345nm, Emission= 445nm; allowing for a real-time read out of 20S immunoproteasome specific activity.

Product Type: Kit

Specificity: Readout: Endpoint/Kinetic; Label or Dye: 7-Amino-4-methylcoumarin; Detection Method: Fluorescence; Substrate Properties: Protein-based Substrate.; Excitation/Emission (nm): 345/445

Crossreactivity: Human

Kit Contains: 10x 20S Immunoproteasome; 50x LLVY-AMC, Chemotrypsin-like activity; 50x PAL-AMC, beta1i/PSMB9 specific substrate; 50x ANW-AMC, beta5i/PSMB8 specific substrate; 50x Inhibitor (ONX-0914, 2mM) in 100% DMSO; 10x Reaction Buffer; 50x SDS(1.75%) in H20; 100x free AMC Standard (40µM)

Short Term Storage: -20°C

Long Term Storage: -80°C

Shipping: DRY ICE

Handling Advice Aliquot to avoid freeze/thaw cycles. Avoid freeze/thaw cycles.

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