ADP Separopore® (Agarose) 4B-CL (Lyophilized)

Applications:

ADP-Separopore 4B-CL is used for the purification of ADP-dependent enzymes and proteins. ADP-Separopore 4B-CL interacts strongly with NADP⁺-dependent dehydrogenases. Selective elution with gradients of NAD⁺ or NADP⁺ allows the resolution of complex mixtures of dehydrogenase isoenzymes using ADP Separopore 4B-CL.

Technical Specifications:

• Ligand: Adenosine 5'-diphosphate (ADP)
• Matrix: Separopore 4B-CL (crosslinked agarose beads, 4%)
• Particle size range: 53 - 180 µm
• Molecular weight range: 6 x 10⁴ - 2 x 10⁷
• Matrix activation: Cyanogen bromide
• Ligand density: ~ 2 μmol of 2’5’-ADP / ml drained gel
• Spacer: 11 atoms (adipic acid dihydrazide)
• Matrix attachment: Ribose hydroxyls
• Binding capacity: >0.4 mg G6PDH / ml drained gel
• pH stability: 4 - 9
• Flow specifications: 70 - 140 cm / h
• Supplied as a lyophilized powder

References:

• Purification of rat kidney glucose 6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, and glutathione reductase enzymes using 2',5'-ADP Sepharose 4B affinity in a single chromatography step. Protein Expr Purif. (2012) 81: 1-4.
• In vitro effects of some drugs on human erythrocyte glutathione reductase. J Enzyme Inhib Med Chem. (2012) 27: 18-23.
• Biochemical comparison of Anopheles gambiae and human NADPH P450 reductases reveals different 2'-5'-ADP and FMN binding traits. PLoS One. (2011) 6: e20574.
• Effects of some drugs on human erythrocyte glucose 6-phosphate dehydrogenase: an in vitro study. J Enzyme Inhib Med Chem. (2010) 25: 871-5.
• Characterization of Deinococcus radiophilus thioredoxin reductase active with both NADH and NADPH. J Microbiol. (2010) 48: 637-43.
• Mitochondrial thioredoxin reductase purification, inhibitor studies, and role in cell signaling. Methods Enzymol. (2010) 474: 109-22.
• Effects of some drugs on human erythrocyte 6-phosphogluconate dehydrogenase: an in vitro study. J Enzyme Inhib Med Chem. (2010) 25: 476-9.
• Purification and characterization of a glutathione reductase from Phaeodactylum tricornutum. Protist. (2010) 161: 91-101.
• Dantrolene inhibits human erythrocyte glutathione reductase. Biol Pharm Bull. (2008) 31: 2036-9.
• Effects of some analgesic anaesthetic drugs on human erythrocyte glutathione reductase: an in vitro study. J Enzyme Inhib Med Chem. (2009) 24: 420-4.
• Investigation of the effects of some sulfonamide derivatives on the activities of glucose-6-phosphate dehydrogenase, 6-phospho gluconate dehydrogenase and glutathione reductase from human erythrocytes. J Enzyme Inhib Med Chem. (2008) 23: 418-23.
• Purification and characterization of glutathione reductase from rainbow trout (Oncorhynchus mykiss) liver and inhibition effects of metal ions on enzyme activity. Comp Biochem Physiol C Toxicol Pharmacol. (2008) 148: 117-21.
• Effects of some antibiotics on human erythrocyte glutathione reductase: an in vitro study. J Enzyme Inhib Med Chem. (2008) 23: 144-8.
• Effects of some metal ions on human erythrocyte glutathione reductase: an in vitro study. Protein Pept Lett. (2007) 14: 1027-30.
• Purification and characterization of glucose 6-phosphate dehydrogenase from Lake Van fish (Chalcalburnus tarichii pallas, 1811) liver. J Physiol Biochem. (2006) 62: 155-61.
• Purification and kinetic properties of glutathione reductase from bovine liver. Mol Cell Biochem. (2007) 303: 45-51.
• Inhibitory effects of ofloxacin and cefepime on enzyme activity of 6-phosphogluconate dehydrogenase from chicken liver. Protein Pept Lett. (2007) 14: 197-207.
• Effects of some drugs on hepatic glucose 6-phosphate dehydrogenase activity in Lake Van fish (Chalcalburnus tarischii Pallas, 1811). J Hazard Mater. (2007) 143: 415-8.

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Usage : bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.