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10 mg

Ref. BWG-21510856-2
BIOWORLD

photos non contractuelles

Neuf 518.00 H.T.
en stock

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Détails Produit

Product Description
Lectin AAL is isolated from orange peel fungus (Aleuria aurantia). AAL is a diamer of two identical subunits with a molecular weight of 72,000. Each subunit has five carbohydrate-binding sites that recognize and bind specifically to fucose and terminal fucose residues on complex oligosaccharides and glycoconjugates. AAL shows high affinity for Fucα6GlcNAc in the core of N-glycans and gives an elution of 100 mM L-fucose. AAL also reversibly binds fucose attached to nucleic acids. This lectin has an isoelectric point of pH 9 and non-specific blood group.

AAL comes in lyophilized powder form and is stable for more than three years from production date when stored below -20˚C. AAL is available in chromatographically purified, affinity purified and conjugated to various fluorophores, biotin, HRP and immobilized to Separopore (agarose) matrix.


Applications:
Studies of glycoproteins and glycolipids
Purification of membrane proteins and receptors
Affinity chromatography
Subcellular localization (Histochemistry)
Agglutination studies
ELISA assays

References

  • Hashimoto S, Asao T, Takahashi J, Yagihashi Y, Nishimura T, Saniabadi AR, Poland DC, van Dijk W, Kuwano H, Kochibe N, Yazawa S. α1-acid glycoprotein fucosylation as a marker of carcinoma progression and prognosis. Cancer. (2004) 101:2825-36.
  • Wimmerova M, Mitchell E, Sanchez JF, Gautier C, Imberty A. Crystal structure of fungal lectin: six-bladed beta-propeller fold and novel fucose recognition mode for Aleuria aurantia lectin. J Biol Chem. (2003) 278:27059-67.
  • Yazawa S, Kochibe N, Asao T. A simple procedure for isolation of tumor-associated antigens by affinity chromatography using fucose-specific Aleuria aurantia lectin. Immunol Invest. (1990) 19:319-27.