Amaranthus caudatus Lectin (ACL/ACA) - Biotinylated

Amaranthus caudatus lectin - Biotinylated

SYNONYMS: Amaranthus caudatus Agglutinin; ACL; ACA

CATEGORY: Bioconjugates > Lectins

CAS#: NA

Uniprot ID: Q71QF2

PRODUCT DESCRIPTION:

Lectins are proteins or glycoproteins of non-immune origin that agglutinate cells and/or precipitate complex carbohydrates. Lectins are capable of binding glycoproteins even in presence of various detergents. Amaranthus caudatus Lectin (ACL) is a homo-dimer composed of two 35 kDa subunits. Isoelectric focusing of the lectin resolves it into four bands with isoelectric points between pH 6.7 and pH 7.7. Unlike most lectins, it binds best in mildly acidic conditions. ACL also does not appear to require divalent cations for activity and tolerates sialic acid substitution at the 3 position of galactose in the “T” antigen.

Biotinylated Amaranthus caudatus lectin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium sodium azide. The biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System.

Technical Specifications:

Ligand Source: Amaranthus caudatus

Molecular Weight: 66-70 kDa

Carbohydrate Specificity: GalBeta1,4GalNAc

Inhibiting/Eluting Sugar: Desialyzed Fetuin

Carbohydrate for Elution: 0.2M Desialyzed Fetuin

Blood Group Specificity: O>B>A (-SA), T antigen

Conjugate: Biotinylated

Form: Liquid

Isoelectric (pI): 6.7-7.7

Storage: -20 C

Application note: N/A

APPLICATIONS:

• Used as a probe for T- and cryptic T-antigens
• Used as a histochemical probe for prolifereating colonic epithelial cells.

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