Amaranthus caudatus Lectin (ACL/ACA) - Separopore® 4B

Separopore conjugated lectin: ACL, ACA (Amaranthus caudatus) Tassel Flower lectin.

ACL (ACA) lectin is a 66 kDa to 70 kDa dimer composed of two subunits of about 35 kDa. Although this lectin preferentially binds to oligosaccharides containing the galactosyl (Beta-1,3) N-acetylgalactosamine structure (T-antigen), tissue staining patterns are markedly different than those obtained with either peanut agglutinin or Jacalin. Amaranthus caudatus lectin also appears to tolerate sialic acid substitution at the 3 position of galactose in the “T” antigen. Used for purification of various glyco conjugates and glycoproteins.

Technical Specifications:

• Ligand source: Amaranthus caudatus
• Matrix: Separopore 4B-CL (crosslinked agarose beads, 4%)
• Particle size range: 52 - 165 µm
• Matrix activation: Cyanogen bromide
• Matrix attachment: Amino
• Ligand density: 5 mg Tassel Flower lectin/ml drained gel.
• Binding Capacity:2-3 mg/mL
• Inhibiting/Eluting Sugar: Desialyzed Fetuin
• Carbohydrate for Elution: .2M Desialyzed Fetuin
• Blood Group Specificity: N/A
• Form: 50:50 Liquid Suspension, PBS (1x) pH 7.4 w/ 0.01% Thimerosal
• pH stability: 4 - 9
• Storage: 2 - 8 C DO NOT FREEZE
• Application note:Not Available