anti-4-Hydroxy-2-nonenal [4-HNE], mAb (HNEJ-2)

Product description: Monoclonal Antibody. Recognizes 4-HNE-lysine, 4-HNE-histidine and 4-HNE cysteine adduct. Has much higher affinity for the 4-HNE-histidine adduct than 4-HNE-lysine or 4-HNE cysteine adduct. Shows almost negligible reactivity with proteins that were treated with other aldehydes, such as 2-nonenal, 2-hexenal, 1-hexanal, 4-hydroxy-2-hexenal, formaldehyde, or glutaraldehyde. Lyophilized. Contains 50mM Tris-buffered saline (TBS). Reactive oxygen species (ROS) are involved in lipid peroxidation inside the living bodies, and membrane lipids are one of the major targets of ROS. During the peroxidation process, a variery of aldehydes are formed. 4-hydroxy-2-nonenal (4-HNE) is an alpha, beta unsaturated aldehyde that can be formed by peroxidation of omega-6 unsaturated fatty acids such as linoleic acid and arachidonic acid. Especially, 4-HNE formed inside living body is reported to originate from phospholipid-bound arachidonic acid. 4-HNE may be one of the most reliable biomarker of lipid peroxidation.

Alternate Names/Synonyms:

Product Type: Monoclonal Antibody

Clone: HNEJ-2

Isotype: Mouse IgG1kappa

Immunogen: 4-Hydroxy-2-nonenal (4-HNE) modified keyhole limpet hemocyanin.

Applications: IHC, WB

Species Crossreactivity: All, Human

Specificity: Recognizes 4-HNE-lysine, 4-HNE-histidine and 4-HNE cysteine adduct. Has much higher affinity for the 4-HNE-histidine adduct than 4-HNE-lysine or 4-HNE cysteine adduct. Shows almost negligible reactivity with proteins that were treated with other aldehydes, such as 2-nonenal, 2-hexenal, 1-hexanal, 4-hydroxy-2-hexenal, formaldehyde, or glutaraldehyde.

Formulation: Lyophilized. Contains 50mM Tris-buffered saline (TBS).

Short Term Storage: +4°C

Long Term Storage: -20°C

Shipping: BLUE ICE

Use & Stability: Stable for at least 3 years after receipt when stored at -20°C. After reconstitution, prepare aliquots and store at -20°C.

Lien vers la Fiche Technique

Literature References: The monclonal antibody specific for the 4-hydroxy-2-nonenal histidine adduct: S.Toyokuni, et al.; FEBS Lett. 359, 189 (1995) [Western blot] | Induction and nuclear translocation of thioredoxin by oxidative damage in the mouse kidney: independence of tubular necrosis and sulfhydryl depletion: T. Tanaka, et al.; Lab. Invest. 77, 145 (1997) | Click here for more literature references! http://www.jaica.com/e/technical_forum.html#4HNE_AB_REF