anti-8-Hydroxy-2'-deoxyguanosine [8-OHdG], mAb (N45.1)

Product description: 8-hydroxy-2'-deoxyguanosine (8-OHdG) is a modified base that occurs in DNA due to attack by hydroxyl radicals (incl. singlet oxygen and direct photodynamic action) that are formed as byproducts and intermediates of aerobic metabolism and during oxidative stress. There is increasing evidence to support the involvement of free radical reactions in the damage of biomolecules that eventually lead to several diseases in humans, such as atherosclerosis, cerebral and heart ischemia-reperfusion injury, cancer, rheumatoid arthritis, inflammation, diabetes, aging, and neurodegenerative conditions, such as Alzheimer’s disease. 8-OHdG is popular as a sensitive, stable and integral marker of oxidative damage in cellular DNA and can be detected in tissue, serum, urine and other biomaterials.

Product Type: Monoclonal Antibody

Clone: N45.1

Isotype: Mouse IgG1kappa

Immunogen: 8-Hydroxy-2’-deoxyguanosine (8OHdG) conjugated keyhole limpet hemocyanin.

Applications: ELISA, IHC

Species Crossreactivity: All, Human

Specificity: Recognizes 8-OHdG. Does not cross-react with 19 analogues of 8-OHdG (guanosine (G), 7-methylG, 6-SHG, 8-bromoG, dA, dC, dT, dI, dU, dG, O6-methyldG, 8-OHdA, guanine (Gua), O6-methylGua, 8-OHGua, uric acid, urea, creatine, creatinine). Minimal cross-reactivity with 8-sulfhydrylG and 8-OHG (less than 1%).

Formulation: Lyophilized. Contains 10mM PBS, pH7.4 containing 1.0% BSA.

Long Term Storage: -20°C

Shipping: BLUE ICE

Use & Stability: Stable for at least 3 years after receipt when stored at -20°C. After reconstitution, prepare aliquots and store at -20°C.

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