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Product description: Monoclonal Antibody. Recognizes 8-OHdG. Does not cross-react with 19 analogues of 8-OHdG (guanosine (G), 7-methylG, 6-SHG, 8-bromoG, dA, dC, dT, dI, dU, dG, O6-methyldG, 8-OHdA, guanine (Gua), O6-methylGua, 8-OHGua, uric acid, urea, creatine, creatinine). Minimal cross-reactivity with 8-sulfhydrylG and 8-OHG (less than 1%). Lyophilized. Contains 10mM PBS, pH7.4 containing 1.0% BSA. 8-hydroxy-2'-deoxyguanosine (8-OHdG) is a modified base that occurs in DNA due to attack by hydroxyl radicals (incl. singlet oxygen and direct photodynamic action) that are formed as byproducts and intermediates of aerobic metabolism and during oxidative stress. There is increasing evidence to support the involvement of free radical reactions in the damage of biomolecules that eventually lead to several diseases in humans, such as atherosclerosis, cerebral and heart ischemia-reperfusion injury, cancer, rheumatoid arthritis, inflammation, diabetes, aging, and neurodegenerative conditions, such as Alzheimer's disease. 8-OHdG is popular as a sensitive, stable and integral marker of oxidative damage in cellular DNA and can be detected in tissue, serum, urine and other biomaterials.
Product Type: Monoclonal Antibody
Isotype: Mouse IgG1kappa
Immunogen: 8-Hydroxy-2-deoxyguanosine (8OHdG) conjugated keyhole limpet hemocyanin.
Applications: ELISA, IHC
Species Crossreactivity: All, Human
Specificity: Recognizes 8-OHdG. Does not cross-react with 19 analogues of 8-OHdG (guanosine (G), 7-methylG, 6-SHG, 8-bromoG, dA, dC, dT, dI, dU, dG, O6-methyldG, 8-OHdA, guanine (Gua), O6-methylGua, 8-OHGua, uric acid, urea, creatine, creatinine). Minimal cross-reactivity with 8-sulfhydrylG and 8-OHG (less than 1%).
Formulation: Lyophilized. Contains 10mM PBS, pH7.4 containing 1.0% BSA.
Short Term Storage: +4°C
Long Term Storage: -20°C
Shipping: BLUE ICE
Use & Stability: Stable for at least 3 years after receipt when stored at -20°C. After reconstitution, prepare aliquots and store at -20°C.
Literature References: Quantitative immunohistochemical determination of 8-hydroxy-2'deoxyguanosine by a monoclonal antibody N45.1: Its application to ferric nitrilotriacetate-induced renal carcinogenesis model: S. Toyokuni, et al.; Lab. Invest. 76, 365 (1997) [Specifity of the antibody clone N45.1] | 8-Hydroxy-2'deoxyguanosine is increased in epidermal cells of hairless mice after chronic ultraviolet B exposure: Y. Hattori, et al.; J. Invest. Dermatol. 107, 733 (1996) [Immunohistochemistry of UV-B irradiated mouse skin] | Induction and nuclear translocation of thioredoxin by oxidative damage in the mouse kidney: independence of tubular necrosis and sulfhydryl depletion: T. Tanaka, et al.; Lab. Invest. 77, 145 (1997) [Immunohistochemistry of ferric nitrotriacetate-treated mouse kidney] | Hyperglycemia causes oxidative stress in pancreatic -cells of GK rats, a model of type 2 diabetes: Y. Ihara, et al.; Diabetes 48, 927 (1999) [Immunohistochemistry of pancreatic islets from GK rat, a diabetes model] | DNA oxidation injury in bone early after steroid administration is involved in the pathogenesis of steroid-induced osteonecrosis: T. Ichiseki, et al.; Rheumatology 44, 456 (2005) [Immunohistochemistry of rabbit tissue] | Protective effect of persimmon (Diospyros kaki) peel proanthocyanidin against oxidative damage under H2O2 -induced cellular senescence: Y.A. Lee, et al.; Biol. Pharm. Bull. 31, 1265 (2008) [Application to human cultured cells] | Click here for more literature references! http://www.jaica.com/e/technical_forum_8ohdg.html#8OHdG_AB_REF
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