anti-Caspase-11 (mouse), mAb (4E11)

Product description: Monoclonal Antibody. Recognizes mouse and rat full-length caspase-11 protein. Detects two bands of ~38kDa and ~43kDa by Western blot. Isotype: Rat IgG1kappa. Clone: 4.00E+11. Applications: ELISA, FACS, IP, WB. Liquid. In PBS containing 0.02% sodium azide. Caspase-11 not only activates caspase-1, that is required for the maturation of proinflammatory cytokines such as interleukin-1 (IL-1) and interleukin-18 (IL-18), but also activates caspase-3, leading to cellular apoptosis under pathological conditions. In most cells, caspase-11 is only expressed upon induction with pro-inflammatory stimuli. Caspase-11 is an essential mediator of endotoxic shock and caspase-11-deficient mice are resistant to endotoxic shock. LPS-induced caspase-11 regulates lymphocyte apoptosis by activating both caspase-3 and caspase-7.

Alternate Names/Synonyms: Caspase-11 (mouse); Protease ICH-3 (mouse)

Product Type: Monoclonal Antibody

Clone: 4E11

Isotype: Rat IgG1kappa

Immunogen: Recombinant mouse caspase-11 (p20 fragment).

Applications: ELISA, FACS, IP, WB

Species Crossreactivity: Mouse, Rat

Specificity: Recognizes mouse and rat full-length caspase-11 protein. Detects two bands of ~38kDa and ~43kDa by Western blot.

Purity: >95% (SDS-PAGE)

Endotoxin: <0.1EU/µg purified protein (LAL test; Lonza).

Concentration: 1mg/ml

Formulation: Liquid. In PBS containing 0.02% sodium azide.

Short Term Storage: +4°C

Long Term Storage: -20°C

Shipping: BLUE ICE

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C.

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Literature References: Apaf-1 and caspase-9 are required for cytokine withdrawal-induced apoptosis of mast cells but dispensable for their functional and clonogenic death: V.S. Marsden, et al.; Blood 107, 1872 (2006) | Apoptosis and non-inflammatory phagocytosis can be induced by mitochondrial damage without caspases: M.F. Van Delft, et al.; Cell Death Differ. 17, 821 (2010)