anti-Caspase-4 /11 (p20), mAb (Flamy-1) (Biotin)

Product description: Inflammatory caspases (also known as group I caspases) are encoded by three main genes in humans, caspase-1, caspase-4 and caspase-5 and three main genes in mouse, caspase-1, caspase-11 and caspase-12. Caspase-11 is the murine orthologue of human caspase-4 and -5. Murine caspase-11 is a poorly characterized member of the caspase-1 subfamily. Caspase-11-deficient embryonic fibroblasts are resistant to apoptosis induced by ectopic expression of caspase-1, suggesting that caspase-11 is an upstream activator of caspase-1. Unlike caspase-1, the expression of caspase-11 is LPS-inducible and it is reasonable to postulate that other members of the family are regulated at the transcriptional or translational level by extracellular stimuli. Recently caspase-11 has been shown to be required for activation of the Nlrp3 inflammasome upon E. coli, V. cholerae and C. rodentium infection. The upstream activators of caspase-11 are TLR4 and TRIF, that modulate enteropathogen-induced inflammasome activation by promoting caspase-11 expression and activation. Formation of ASC foci (specks), a measure of NLRP3/ASC complex formation, requires caspase-11 but not caspase-1 indicating that caspase-11 acts upstream of the NLRP3/ASC complex.

Alternate Names/Synonyms: Caspase-11 (mouse); Caspase-4 (human); ICE(rel)-II (human); Protease ICH-2 (human); Protease TX (human); Protease ICH-3 (mouse)

Product Type: Monoclonal Antibody

Clone: Flamy-1

Isotype: Mouse IgG2bkappa

Immunogen: Recombinant mouse Caspase-11 (aa 81-373).

Labels - Conjugates: Biotin

Applications: IP, WB

Species Crossreactivity: Human, Mouse

Specificity: Recognizes endogenous full-length protein and activated (p20) fragment of mouse and human caspase-4/11.

Concentration: 1mg/ml

Formulation: Liquid. In PBS containing 0.02% sodium azide.

Long Term Storage: -20°C

Shipping: BLUE ICE

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C.

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