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100 µg

Ref. AG-20T-0137-C100

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Neuf 280.00 H.T.


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Détails Produit

Product description: Monoclonal Antibody. Recognizes the p20 subunit of mouse caspase-8. Detects bands of ~55kDa (full-length caspase-8) and ~18kDa (apoptosis-induced cleavage fragment) by Western blot. Does not cross-react with human caspase-8. Isotype: Rat IgG1kappa. Clone: 1G12. Applications: ELISA, FACS, IHC, WB. Liquid. In PBS containing 0.02% sodium azide. Procaspase-8 belongs to the family of caspases. Binding of FasL to Fas leads to formation of a receptor complex at the cellular membrane, which was named DISC. The DISC consists of oligomerized receptors, the DD-containing adaptor molecule FADD, procaspase-8, procaspase-10 and c-FLIP. The DISC structure provides a platform for the oligomerization of procaspase-8 that allows two procaspase-8 homodimers to be in the close proximity leading to the initial activation of procaspase-8. At the first cleavage step, the N-terminal p43/p41 and the C-terminal p30 cleavage products are generated. Importantly, these cleavage products already possess catalytic activity. At the second cleavage step, p43/p41 and p30 are processed to p10 and p20, respectively, which leads to the generation of the active caspase-8 heterotetramer (p20/p10)2.

Alternate Names/Synonyms: Apoptotic Cysteine Protease; Apoptotic Protease Mch-5; CAP4; FADD-homologous ICE/ced-3-like Protease

Product Type: Monoclonal Antibody

Clone: 1G12

Isotype: Rat IgG1kappa

Immunogen: Recombinant mouse caspase-8 (p20 subunit).

Applications: ELISA, FACS, IHC, WB

Species Crossreactivity: Mouse

Specificity: Recognizes the p20 subunit of mouse caspase-8. Detects bands of ~55kDa (full-length caspase-8) and ~18kDa (apoptosis-induced cleavage fragment) by Western blot. Does not cross-react with human caspase-8.

Purity: >95% (SDS-PAGE)

Endotoxin: <0.1EU/µg purified protein (LAL test; Lonza).

Concentration: 1mg/ml

Formulation: Liquid. In PBS containing 0.02% sodium azide.

Short Term Storage: +4°C

Long Term Storage: -20°C

Shipping: BLUE ICE

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C.

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Literature References: Apaf-1 and caspase-9 do not act as tumor suppressors in myc-induced lymphomagenesis or mouse embryo fibroblast transformation: C.L. Scott, et al.; J. Cell Biol. 164, 89 (2004) | Modifications and intracellular trafficking of FADD/MORT1 and caspase-8 after stimulation of T lymphocytes: L.A. O'Reilly, et al.; Cell Death Differ. 11, 724 (2004) | Caspase-8 serves both apoptotic and nonapoptotic roles: T.B.Kang, et al.; J. Immunol. 73, 2976 (2004) | Role of membrane sphingomyelin and ceramide in platform formation for Fas-mediated apoptosis: M. Miyaji, et al.; J. Exp. Med. 202, 249 (2005) | Fatal Hepatitis Mediated by Tumor Necrosis Factor TNFalpha Requires Caspase-8 and Involves the BH3-Only Proteins Bid and Bim: T. Kaufmann, et al.; Immunity 30, 56 (2009) | Catalytic activity of the caspase-8-FLIPL complex inhibits RIPK3-dependent necrosis: A. Oberst, et al.; Nature 471, 363 (2011) | FLIP (Flice-like inhibitory protein) suppresses cytoplasmic double-stranded-RNA-induced apoptosis and NF-B and IRF3-mediated signaling: P. Handa, et al.; Cell Commun. Signal. 9, 16 (2011) | XIAP restricts TNF- and RIP3-dependent cell death and inflammasome activation: M. Yabal, et al.; Cell Rep. 7, 1796 (2014)