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100 µg

Ref. AG-20T-0138-C100

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Neuf 280.00 H.T.


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Détails Produit

Product description: Monoclonal Antibody. Recognizes the p20 subunit of mouse caspase-8. Detects bands of ~55kDa (full-length caspase-8) and ~18kDa (apoptosis-induced cleavage fragment) by Western blot. Does not cross-react with human caspase-8. Isotype: Rat IgG1kappa. Clone: 3B10. Applications: ELISA, FACS, ICC, IHC, WB. Liquid. In PBS containing 0.02% sodium azide. Procaspase-8 belongs to the family of caspases. Binding of FasL to Fas leads to formation of a receptor complex at the cellular membrane, which was named DISC. The DISC consists of oligomerized receptors, the DD-containing adaptor molecule FADD, procaspase-8, procaspase-10 and c-FLIP. The DISC structure provides a platform for the oligomerization of procaspase-8 that allows two procaspase-8 homodimers to be in the close proximity leading to the initial activation of procaspase-8. At the first cleavage step, the N-terminal p43/p41 and the C-terminal p30 cleavage products are generated. Importantly, these cleavage products already possess catalytic activity. At the second cleavage step, p43/p41 and p30 are processed to p10 and p20, respectively, which leads to the generation of the active caspase-8 heterotetramer (p20/p10)2.

Alternate Names/Synonyms: Apoptotic Cysteine Protease; Apoptotic Protease Mch-5; CAP4; FADD-homologous ICE/ced-3-like Protease

Product Type: Monoclonal Antibody

Clone: 3B10

Isotype: Rat IgG1kappa

Immunogen: Recombinant mouse caspase-8 (p20 subunit).

Applications: ELISA, FACS, ICC, IHC, WB

Species Crossreactivity: Mouse

Specificity: Recognizes the p20 subunit of mouse caspase-8. Detects bands of ~55kDa (full-length caspase-8) and ~18kDa (apoptosis-induced cleavage fragment) by Western blot. Does not cross-react with human caspase-8.

Purity: >95% (SDS-PAGE)

Endotoxin: <0.1EU/µg purified protein (LAL test; Lonza).

Concentration: 1mg/ml

Formulation: Liquid. In PBS containing 0.02% sodium azide.

Short Term Storage: +4°C

Long Term Storage: -20°C

Shipping: BLUE ICE

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C.

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Literature References: Modifications and intracellular trafficking of FADD/MORT1 and caspase-8 after stimulation of T lymphocytes: L.A. O'Reilly, et al.; Cell Death Differ. 11, 724 (2004) | Stimulation of Toll-like receptor 3 and 4 induces interleukin-1 maturation by caspase-8: J. Maelfait, et al.; J. Exp. Med. 205, 1967 (2008) | Impaired lactation in mice expressing dominant-negative FADD in mammary epithelium: M. Shackleton, et al.; Dev. Dyn. 238, 1010 (2009) | Caspase-8 mediates mitochondrial release of pro-apoptotic proteins in a manner independent of its proteolytic activity in apoptosis induced by the protein synthesis inhibitor acetoxycycloheximide in human leukemia Jurkat cells: K. Kadohara, et al.; J. Biol. Chem. 284, 5478 (2009) | Cytotoxic T lymphocyte perforin and Fas ligand working in concert even when Fas ligand lytic action is still not detectable: D. Hassin, et al.; Immunology 133, 190 (2011)