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20 µg

Ref. JAI-MDT-020P
JAICA

photos non contractuelles

Neuf 191.00 H.T.

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Détails Produit

Product description: Monoclonal Antibody. Recognizes free dityrosine, 3-(p-hydroxyphenyl) propionic acid dimer, dityrosine-BSA conjugate, and dityrosine in protein or peptides. Does not cross-react with nitrotyrosine, chlorotyrosine, aminotyrosine, phosphotyrosine, tyrosine, 3-(p-hydroxyphenyl) propionic acid, phenylalanine, 3,4-dihydroxyphenylacetic acid (DOPA). Lyophilized. Contains 10mM PBS (pH7.4). Tyrosine is one of the major targets of protein oxidation. Dityrosine (DT) is known to be formed when tyrosine is damaged by free radicals, such as reactive oxygen species (ROS), metal-catalyzed oxidation, ultraviolet irradiation, and peroxidases. Dityrosine has been found in atherosclerotic lesions, and lipofuscin of pyramidal neurons of aged human brains. Dityrosine is a specific biomarker for protein oxidation. Dityrosine is reported to exist in various tissues and was also recently detected in urine samples. It is expected to be a novel protein oxdation marker, which is non-invasively detectable.

Alternate Names/Synonyms:

Product Type: Monoclonal Antibody

Clone: 1C3

Isotype: Mouse IgG2akappa

Immunogen: 3-(p-hydroxyphenyl) propionic acid dimer-conjugated keyhole limpet hemocyanin.

Applications: ELISA, IHC, WB

Species Crossreactivity: All, Human

Specificity: Recognizes free dityrosine, 3-(p-hydroxyphenyl) propionic acid dimer, dityrosine-BSA conjugate, and dityrosine in protein or peptides. Does not cross-react with nitrotyrosine, chlorotyrosine, aminotyrosine, phosphotyrosine, tyrosine, 3-(p-hydroxyphenyl) propionic acid, phenylalanine, 3,4-dihydroxyphenylacetic acid (DOPA).

Formulation: Lyophilized. Contains 10mM PBS (pH7.4).

Short Term Storage: +4°C

Long Term Storage: -20°C

Shipping: BLUE ICE

Use & Stability: Stable for at least 3 years after receipt when stored at -20°C.

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Literature References: Immunochemical detection of protein dityrosine in atherosclerotic lesion of apo-E-deficient mice using a novel monoclonal antibody: Y. Kato, et al.; BBRC 275, 11 (2000) | The hydrogen peroxide/copper ion system, but not other metal-catalyzed oxidation systems, produces protein-bound dityrosine: Y. Kato, et al.; Free Radic. Biol. Med. 31, 624 (2001) | Copper mediates dityrosine cross-linking of Alzheimer’s amyloid-beta: C.S. Atwood, et al.; Biochemistry 43, 560 (2004) | Immunohistochemical detection of oxidative stress biomarkers, dityrosine and N(epsilon)-(hexanoyl)lysine, and C-reactive protein in rabbit atherosclerotic lesions: Y. Fukuchi, et al.; J. Atheroscler. Thromb. 15, 185 (2008) | Expressions of protein oxidation markers, dityrosine and advanced oxidation protein products in Cisplatin-induced nephrotoxicity in rats: Y. Kimoto, et al.; J. Vet. Med. Sci. 73, 403 (2011) | Effect of protein malnutrition on the skin epidermis of hairless mice: A. Sugiyama, et al.; J. Vet. Med. Sci. 73, 831 (2011) | Expressions of protein oxidation markers, dityrosine and advanced oxidation protein products in acetaminophen-induced liver injury in rats: J. Sun, et al.; J. Vet. Med. Sci. 73, 1185 (2011) | Intramolecular cross-linking in the native JHBP molecule: D. Bystranowska, et al.; Arch. Biochem. Biophys. 517, 12 (2012) | Di-tyrosine cross-link decreases the collisional cross-section of Ab peptide dimers and trimers in the gas phase: An ion mobility study: E. Sitkiewicz, et al.; PLOS One 9, e100200 (2014)