AVANTI POLAR LIPIDS , CYGNUS , LARODAN , ADIPOGEN , MYBIOSOURCE , SERVA NORDMARK, BIOWORLD, EDGEBIO , PANREAC APPLICHEM
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Product description: Monoclonal Antibody. Recognizes Hexanoyl-Lys adducts. Does not cross-react with MDA, glyoxal, methylglyoxal, 1-hexanal, 2-hexenal, 1-nonannal, 2-nonenal, 4-hudroxy-2-nonenal. Lyophilized. Contains 10mM PBS (pH7.4), 5% sucrose, 1% BSA and 0.05% Procline 950. Oxidative damage to lipids (lipid peroxidation) has been found to play an important role in various disease and aging processes. During early stages of lipid peroxidation, lipid hydroperoxides (LOOH) are formed. These can react additionally to form later stage end products such as malondialdehyde (MDA) and hydroxynonenal (HNE). LOOH is measured to quantify early stage or acute lipid peroxidation while MDA is commonly measured to quantify late stage or chronic lipid peroxidation. More recently, it has been reported that 13-hydroperoxyoctadecanoic acid (13-HPODE), a precursor to 13-hydroxyoctadecanoic acid (13-HODE) can react with proteins to form measurable adducts by covalently binding to specific amino acid residues. The Hexanoyl-Lysine (HEL) adduct is formed upon oxidative modification of omega-6 fatty acids such as linoleic acid, the predominant polyunsaturated fatty acid (PUFA) in the human diet, and arachidonic acid. HEL may be another useful biomarker for detecting and quantifying the earlier stages of lipid peroxidation.
Product Type: Monoclonal Antibody
Isotype: Mouse IgG1kappa
Immunogen: N epsilon hexanoyl-keyhole limpet hemocyanin (HEL-KLH).
Applications: ELISA, IHC, WB
Species Crossreactivity: All, Human
Specificity: Recognizes Hexanoyl-Lys adducts. Does not cross-react with MDA, glyoxal, methylglyoxal, 1-hexanal, 2-hexenal, 1-nonannal, 2-nonenal, 4-hudroxy-2-nonenal.
Formulation: Lyophilized. Contains 10mM PBS (pH7.4), 5% sucrose, 1% BSA and 0.05% Procline 950.
Short Term Storage: +4°C
Long Term Storage: -20°C
Shipping: BLUE ICE
Use & Stability: Stable for at least 3 years after receipt when stored at -20°C. After reconstitution, prepare aliquots and store at -20°C.
Literature References: Preparation of a monoclonal antibody to N(epsilon)-(Hexanonyl)lysine: application to the evaluation of protective effects of flavonoid supplementation against exercise-induced oxidative stress in rat skeletal muscle: Y. Kato, et al.; BBRC 274, 389 (2000) [Development and characterization of anti-HEL monoclonal antibody] | Immunohistochemical detection of oxidative stress biomarkers, dityrosine and N(epsilon)-(hexanoyl)lysine, and C-reactive protein in rabbit atherosclerotic lesions: Y. Fukuchi, et al.; J. Atheroscler. Thromb. 15, 185 (2008) [Application to rabbit atherosclerotic lesions] | A water-soluble fullerene vesicle alleviates angiotensin II-induced oxidative stress in human umbilical venous endothelial cells: R. Maeda, et al.; Hypertens. Res. 31, 141 (2008) [Application to cultured cells (HUVECs)] | Differential Effects of High Glucose and Methylglyoxal on Viability and Polyol Metabolism in Immortalized Adult Mouse Schwann Cells: K. Sango, et al.; Open Diabetes J. 1, 1 (2008) [Application to cultured cell from mouse]
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