anti-NLRP3/NALP3, mAb (Cryo-2)

Product description: Monoclonal Antibody. Recognizes mouse and human NLRP3/NALP3. Isotype: Mouse IgG2b. Clone: Cryo-2. Applications: IHC, IP, WB. Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. The inflammasome is a multiprotein complex that mediates the activation of caspase-1, which promotes, amongst others, the secretion of the proinflammatory cytokines interleukin (IL)-1beta and IL-18. Members of the Nod-like receptor family, including NLRP3/NALP3, are critical components of the inflammasome that link danger-signals to caspase-1 activation. Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1), Muckle-Wells syndrome (MWS) and of chronic infantile neurologic cutaneous and articular syndrome (CINCA).

Alternate Names/Synonyms: NACHT-, LRR- and PYD-containing Protein 3; NLRP3; Cryopyrin; Cold Autoinflammatory Syndrome 1 Protein

Product Type: Monoclonal Antibody

Clone: Cryo-2

Isotype: Mouse IgG2b

Immunogen: Recombinant mouse NLRP3/NALP3 (pyrin domain/aa 1-93).

Applications: IHC, IP, WB

Species Crossreactivity: Human, Mouse

Specificity: Recognizes mouse and human NLRP3/NALP3.

Purity: >95% (SDS-PAGE)

Concentration: 1mg/ml

Formulation: Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.

Short Term Storage: +4°C

Long Term Storage: -20°C

Shipping: BLUE ICE

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C.

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Literature References: Thioredoxin-interacting protein links oxidative stress to inflammasome activation: R. Zhou, et al.; Nature Immunology 11, 136 (2010) | The NLRP3 inflammasome promotes renal inflammation and contributes to CKD: A. Vilaysane, et al.; J. Am. Soc. Nephrol. 21, 1732 (2010) | A role for mitochondria in NLRP3 inflammasome activation: R. Zhou, et al.; Nature 469, 221 (2011) | Differential expression of NLRP3 among hematopoietic cells: G. Guarda, et al.; J. Immunol. 186, 2529 (2011) | Mechanism of Impaired NLRP3 Inflammasome Priming by Monophosphoryl Lipid A: C.A. Embry, et al.; Sci. Signal. 4, ra28 (2011) | Measles Virus V Protein Inhibits NLRP3 Inflammasome-Mediated Interleukin-1 Secretion: N. Komune, et al.; J. Virol. 85, 13019 (2011) | Novel role of PKR in inflammasome activation and HMGB1 release: B. Lu, et al.; Nature 488, 670 (2012) | Deubiquitination of NLRP3 by BRCC3 Critically Regulates Inflammasome Activity: B.F. Py, et al.; Mol. Cell 49, 331 (2012) | NLRP3 is activated in Alzheimer's disease and contributes to pathology in APP/PS1 mice: M.T. Heneka, et al.; Nature 493, 67 (2012) | Activation of autophagy by inflammatory signals limits IL-1b production by targeting ubiquitinated inflammasomes for destruction: C.-S. Shi, et al.; Nat. Immunol. 13, 255 (2012) | Inflammasome-independent NLRP3 augments TGF-beta signaling in kidney epithelium: W. Wang, et al.; J. Immunol. 190, 1239 (2013) | Omega-3 Fatty Acids Prevent Inflammation and Metabolic Disorder through Inhibition of NLRP3 Inflammasome Activation: Y. Yan, et al.; Immunity 38, 1154 (2013) | LRRFIP2 negatively regulates NLRP3 inflammasome activation in macrophages by promoting Flightless-I-mediated caspase-1 inhibition: J. Jin, et al.; Nat. Commun. 4, 2075 (2013) | Proapoptotic Chemotherapeutic Drugs Induce Noncanonical Processing and Release of IL-1beta via Caspase-8 in Dendritic Cells: C. Antonopoulus, et al. J. Immunol. 191, 4789 (2013) | Constitutively activated NLRP3 inflammasome causes inflammation and abnormal skeletal development in mice: S.L. Bonar, et al.; PLoS One 7, e35979 (2012) | FADD and caspase-8 mediate priming and activation of the canonical and noncanonical Nlrp3 inflammasomes: P. Gurung, et al.; J. Immunol. 192, 1835 (2014) | XIAP Restricts TNF- and RIP3-Dependent Cell Death and Inflammasome Activation: M. Yabal, et al.; Cell Rep. 7, 1796 (2014) | The adaptor ASC has extracellular and 'prionoid' activities that propagate inflammation: B.S. Franklin, et al.; Nat. Immunol. 15, 727 (2014) | The NLRP3 inflammasome is released as a particulate danger signal that amplifies the inflammatory response: A. Baroja-Mazo, et al.; Nat. Immunol. 15, 738 (2014) | Toward a chemical marker for inflammatory disease: a fluorescent probe for membrane-localized thioredoxin: M.H. Lee, et al.; JACS 136, 8430 (2014) | Prostaglandin E2 inhibits NLRP3 inflammasome activation through EP4 receptor and intracellular cyclic AMP in human macrophages: M. Sokolowska, et al.; J. Immunol. 194, 5472 (2015) | The receptor NLRP3 is a transcriptional regulator of TH2 differentiation: M. Bruchard, et al.; Nat. Immunol. 16, 859 (2015) | NLRP3 Inflammasome Is Expressed and Functional in Mouse Brain Microglia but Not in Astrocytes: A. Gustin, et al.; PLos One 10, e0130624 (2015) | Nlrp6 promotes recovery after peripheral nerve injury independently of inflammasomes: E. Ydens, et al.; J. Neuroinfl. 12, 143 (2015) | Human monocytes engage an alternative inflammasome pathway: M.M. Gaidt, et al.; Immunity 44, 833 (2016)