anti-PARP-10 [ARTD10] (human), mAb (5H11)

Product description: Monoclonal Antibody. Recognizes human PARP-10 [ARTD10] with an epitope between aa 256-407, containing the glycine rich (G-rich) region. Isotype: Rat IgG1. Clone: 5H11. Applications: ICC, IP, WB. Liquid. Cell culture supernatant containing RPMI, 10% FCS and 0.05% sodium azide. PARP-10 is a mono-ADP-ribosyltransferase and was initially identified as an interaction partner of the proto-oncoprotein Myc. PARP-10 possesses ubiquitin-interaction motifs, which are important to inhibit the activation of NF-kappaB and downstream target genes in response to interleukin-1beta and tumor necrosis factor-alpha. PARP-10 mono(ADP-ribosyl)ates (MARylates) NEMO (NF-kappaB essential modulator), which results in reduced NEMO polyubiquitylation and thus decreased NF-kappaB signaling. Overexpression of PARP-10 was shown to lead to apoptosis. PARP-10 knockdown leads to increased cell survival. Additional substrates of PARP-10, such as GSK-3beta were identified and PARP-10-mediated mono(ADP-ribosyl)ation (MARylation) was shown to be involved in regulating multiple processes, including Wnt signaling.

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