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100 µg

Ref. AG-20B-0020B-C100

photos non contractuelles

Neuf 410.00 H.T.


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Détails Produit

Product description: Monoclonal Antibody. Recognizes the posttranslational modification (poly)glutamylation on proteins. Reacts with polyglutamylated alpha- and beta-tubulin. Isotype: Mouse IgG1kappa. Clone: GT335. Applications: ICC, IHC, IP, WB. Liquid. In PBS containing 0.02% sodium azide. Polyglutamylation is a post-translational modification in which glutamate side chains of variable lengths are formed on the modified protein. It is evolutionarily conserved and the most prominent substrate is tubulin, the microtubule (MT) building block. Polyglutamylation has been proposed to be involved in the functional adaptation of MTs, as it occurs within the carboxy-terminal tubulin tails that participate directly in the binding of many structural and motor MT-associated proteins. The recent identification of new substrates of polyglutamylation indicates that this post-translational modification could be a potential regulator of diverse cellular processes and be involved in cell cycle and cell proliferation.

Alternate Names/Synonyms: Polyglutamylated Tubulin; Glutamylated Tubulin; Postranslational Protein Glutamylation

Product Type: Monoclonal Antibody

Clone: GT335

Isotype: Mouse IgG1kappa

Immunogen: Octapeptide EGEGE*EEG, modified by the addition of two glutamyl units onto the fifth E (indicated by an asterisk).

Labels - Conjugates: Biotin

Applications: ICC, IHC, IP, WB

Species Crossreactivity: All

Specificity: Recognizes the posttranslational modification (poly)glutamylation on proteins. Reacts with polyglutamylated alpha- and beta-tubulin.

Purity: >95% (SDS-PAGE)

Concentration: 1mg/ml

Formulation: Liquid. In PBS containing 0.02% sodium azide.

Short Term Storage: +4°C

Long Term Storage: -20°C

Shipping: BLUE ICE

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C.

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Literature References: Distribution of glutamylated alpha and beta-tubulin in mouse tissues using a specific monoclonal antibody, GT335: A. Wolff, et al.; Eur. J. Cell Biol. 59, 425 (1992) | Polyglutamylation of nucleosome assembly proteins: C. Regnard, et al.; J. Biol. Chem. 275, 15969 (2000) | Glutamylated tubulin: diversity of expression and distribution of isoforms: M.L. Kann, et al.; Cell Motil. Cytoskeleton 55, 14 (2003) | Polyglutamylation Is a Post-translational Modification with a Broad Range of Substrates: J. van Dijk, et al.; J. Biol. Chem. 283, 3915 (2008) | Tubulin detyrosination promotes monolayer formation and apical trafficking in epithelial cells: S. Zink, et al.; J. Cell Sci. 125, 5998 (2012) | Microtubule detyrosination guides chromosomes during mitosis: M. Barisic, et al.; Science 348, 6236 (2015) (Supplement)