anti-Thymidine Glycol [TG], mAb (2E8)

Product description: Monoclonal Antibody. Recognizes DNA containing thymidine glycol. Does not cross-react with oxidized dC polymer, oxidized dG polymer and oxidized dA polymer. Does not react with free thymidine glycol. Lyophilized. Contains 10mM PBS, pH7.4 containing 1.0% BSA. Thymidineglycol (TG) is one of the major oxidation products of DNA. Thymidine (T) can be damaged by oxidative stress such as radiation and energy metabolism. Two different pathways to form TG have been suggested. Deoxythymidine in DNA is directly oxidised by hydroxy radical, to form TG. TG can be also formed through an intermediate thymidine chlorohydrin, which is derived from hypochlorous acid (HOCl) from neutrophil myeloperoxidase. Thymidineglycol is derived from DNA, not from RNA. TG is the oxidative stress marker secific for DNA damage. In comparison with 8-hydroxy-2'-deoxyguanosine (8-OHdG), which is the best known DNA oxidation product it was shown that in a lipopolysaccharide (LPS)-treated mouse liver, 8-OHdG can be stained within 24 hours after LPS treatment. TG can be detected within 6 hours, and remains at least for 72 hours.

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