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5 mL

Ref. BWG-20162019-2
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Détails Produit

SYNONYMS: Peanut agglutinin; PNA; Galactose-binding lectin
CATEGORY: Bioconjugates > Lectins
Uniprot ID: P02872

PRODUCT DESCRIPTION:

Lectins are proteins or glycoproteins of non-immune origin that agglutinate cells and/or precipitate complex carbohydrates. Lectins are capable of binding glycoproteins even in presence of various detergents.

Arachis hypogaea lectin or Peanut Agglutinin (PNA) is isolated from peanuts and purified by affinity chromatography. The lectin has a molecular weight of 110 kDa and consists of four identical subunits of approximately 27 kDa each. PNA does not agglutinate normal human erythrocytes, but strongly agglutinates neuraminidase treated erythrocytes. Lectin PNA is specific for terminal Beta-galactose and binds preferentially to a commonly occurring structure, galactosyl (Beta-1,3) N-acetylgalactosamine. PNA has potent anti-T activity similar to the anti-T antibody in human sera. PNA has been used in tumour tissue determination for transitional mucosa malignancies. The lectin also agglutinates neuraminidase-treated human erythrocytes at <0.1 µg/ml after trypsin treatment of cells and its activity is inhibited by lactose and galactose. Though PNA does not require any divalent cations for activity, the presence of calcium ions in diluents can enhance the binding of PNA to receptors, possibly by neutralizing the negative charges on sialic acid residues adjacent to the receptor sequence.

PNA is useful in distinguishing between normal and tumor tissues and in assessing malignancy in transitional mucosa. In addition, PNA binding can be used to measure cellular maturity in lymphoid tissues, to distinguish a variety of lymphocyte subpopulations in man and experimental animals, and to measure the levels of lymphoid cell populations in many diseases. PNA can be employed in the fractionation of stem cells in mice for use in bone marrow transplantation across histocompatibility barriers.

MagneZoom-PNA is silica-coated super paramagnetic beads covalently linked to Peanut Agglutinin (PNA). Peanut agglutinin is a non-glycosylated lectin with a high binding affinity for the tumor associated Thomsen-Friedenreich antigen (“T’-antigen) and binds preferentially to galactosyl (α-1,3) N-acetylgalactosamine. Presence of Ca2+ can enhance PNA binding.

TECHNICAL SPECIFICATIONS:

Ligand Source: Arachis hypogaea

Matrix: MagneZOOM™ (Paramagnetic Silica Coated Beads)

Particle size range: 2-5 µm

Matrix attachment: Amide

Binding Capacity: 1.8 mg/ ml

Inhibiting/Eluting Sugar: Galactose (200 mM)

Carbohydrate for Elution: .2M Galactose

Blood Group Specificity: T antigen (M, N)

Form: 25 mg/mL , PBS (1x) pH 7.4 w/ 0.01% Thimerosal

pH stability: 4 - 9

Storage: 2 - 8 C, DO NOT FREEZE

Application note: Calcium and Manganese are required for binding.

APPLICATIONS:

  • PNA can be used in detection of relocalization of Tag antigen in large bowel carcinoma
  • PNA can be used to distinguish between human lymphocyte subsets.
  • PNA can be used as probe in histochemistry and immunohistochemistry
  • PNA can be used in human erythrocyte/lymphocyte studies
  • To investigate the changes related to cell differentiation.
  • Agglutinates neuraminidase-treated human erythrocytes.
  • PNA is useful in distinguishing between normal and tumor tissues and in assessing malignancy in transitional mucosa.
  • To measure the levels of lymphoid cell populations in many diseases.

UNIT DEFINITION:

One unit will form 1 mg purpurogallin in 20 sec from pyrogallol at pH 6 at 20 C

REFERENCES:

Requires MagneZoom paramagnetic bead station (20160000-1) for separating beads.

Usage : bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.