Arachis hypogaea Lectin (PNA) - TRITC (Rhodamine)

Arachis hypogaea (Peanut) Lectin - Rhodamine (TRITC) -Conjugated (SKU # 21761001)

SYNONYMS: Peanut Agglutinin; PNA; Galactose-binding Lectin

CATEGORY: Bioconjugates > Lectins

Uniprot ID: P02872

PRODUCT DESCRIPTION:

Lectins are proteins or glycoproteins of non-immune origin that agglutinate cells and/or precipitate complex carbohydrates. Lectins are capable of binding glycoproteins even in presence of various detergents.

Arachis hypogaea lectin or Peanut Agglutinin (PNA) is isolated from peanuts and purified by affinity chromatography. The lectin has a molecular weight of 110 kDa and consists of four identical subunits of approximately 27 kDa each. PNA does not agglutinate normal human erythrocytes, but strongly agglutinates neuraminidase treated erythrocytes. Lectin PNA is specific for terminal Beta-galactose and binds preferentially to a commonly occurring structure, galactosyl (Beta-1,3) N-acetylgalactosamine. PNA has potent anti-T activity similar to the anti-T antibody in human sera. PNA has been used in tumour tissue determination for transitional mucosa malignancies. The lectin also agglutinates neuraminidase-treated human erythrocytes at < 0.1 µg/ml after trypsin treatment of cells and its activity is inhibited by lactose and galactose. Though PNA does not require any divalent cations for activity, the presence of calcium ions in diluents can enhance the binding of PNA to receptors, possibly by neutralizing the negative charges on sialic acid residues adjacent to the receptor sequence.

PNA is useful in distinguishing between normal and tumor tissues and in assessing malignancy in transitional mucosa. In addition, PNA binding can be used to measure cellular maturity in lymphoid tissues, to distinguish a variety of lymphocyte subpopulations in man and experimental animals, and to measure the levels of lymphoid cell populations in many diseases. PNA can be employed in the fractionation of stem cells in mice for use in bone marrow transplantation across histocompatibility barriers.

Rhodamine (TRITC) labeled PNA has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 550 nm and the emission maximum is at 575 nm.

Technical Specifications:

Ligand Source: Arachis hypogaea

Molecular Weight: 110 kDa

Carbohydrate Specificity: GalBeta1,3GalNAc (T-antigen)

Inhibiting/Eluting Sugar: Galactose

Carbohydrate for Elution: 0.2M Galactose

Blood Group Specificity: T antigen (M, N)

Conjugate: Rhodamine

Form: Liquid

Isoelectric (pI): 5.5-6.5

Storage: -20 C

Application note: Calcium and Magnesium are required for binding.

APPLICATIONS

• Biotynylated PNA can be used for the detection of relocalization of Tag antigen in large bowel carcinoma
• Biotynylated PNA can be used to distinguish between human lymphocyte subsets.
• Biotynylated PNA can be used as probe in histochemistry and immuno-histochemistry.
• Biotynylated PNA can be used in human erythrocyte/lymphocyte studies.

UNIT DEFINITION: One unit will form 1mg purpurogallin in 20 sec from pyrogallol at pH 6.0 at 20 C

REFERENCES:

• Intravitreal injection of fluorochrome-conjugated peanut agglutinin results in specific and reversible labeling of mammalian cones in vivo. Invest Ophthalmol Vis Sci. (2008) 49: 2643-50.
• Distribution of glycoconjugates in human testis. A histochemical study using fluorescein- and rhodamine-conjugated lectins. Andrologia. (1987) 19: 322-32.
• Identification of osteoclasts by rhodamine-conjugated peanut agglutinin. Calcif Tissue Int. (1986) 39: 161-5.
• A subset of human cord blood mononuclear cells is similar to Langerhans cells of the skin: a study with peanut agglutinin and monoclonal antibodies. Hum Immunol. (1986) 15: 164-74.
• Lectin staining of rat testis and epididymis after ligation of excurrent ducts at different levels. Biol Reprod. (1985) 33: 477-85.
• Effect of castration on lectin staining in rat epididymis. Andrologia. (1985) 17: 327-37.
• Lectin-binding pattern of bull testis and epididymis. J Androl. (1985) 6: 230-42.
• Two molecularly independent surface receptors identify bovine T lymphocytes. J Immunol Methods. (1981) 45: 209-19.