Plus de 50 ans d’expérience à votre service

Tel. : 01 45 33 67 17 / E-mail : coger@coger.fr

Stock épuisé.
En rupture de stock
Quantité minimum d'achat
La quantité minimum d'achat n'est pas atteinte

 

 

2 mg

Ref. BWG-21761002-1
BIOWORLD

photos non contractuelles

Prix sur demande. Demandez un devis : coger@coger.fr

Alerte réapprovisionnement
Recevez une alerte par email dès que votre choix sera de retour en stock
Votre e-mail*:

Détails Produit

Arachis hypogaea (Peanut) Lectin - Texas Red-Conjugated

SYNONYMS: Peanut Agglutinin; PNA; Galactose-binding Lectin

CATEGORY: Bioconjugates > Lectins

Uniprot ID: P02872

PRODUCT DESCRIPTION:

Lectins are proteins or glycoproteins of non-immune origin that agglutinate cells and/or precipitate complex carbohydrates. Lectins are capable of binding glycoproteins even in presence of various detergents.

Arachis hypogaea lectin or Peanut Agglutinin (PNA) is isolated from peanuts and purified by affinity chromatography. The lectin has a molecular weight of 110 kDa and consists of four identical subunits of approximately 27 kDa each. PNA does not agglutinate normal human erythrocytes, but strongly agglutinates neuraminidase treated erythrocytes. Lectin PNA is specific for terminal Beta-galactose and binds preferentially to a commonly occurring structure, galactosyl (Beta-1,3) N-acetylgalactosamine. PNA has potent anti-T activity similar to the anti-T antibody in human sera. PNA has been used in tumour tissue determination for transitional mucosa malignancies. The lectin also agglutinates neuraminidase-treated human erythrocytes at <0.1 µg/ml after trypsin treatment of cells and its activity is inhibited by lactose and galactose. Though PNA does not require any divalent cations for activity, the presence of calcium ions in diluents can enhance the binding of PNA to receptors, possibly by neutralizing the negative charges on sialic acid residues adjacent to the receptor sequence.

PNA is useful in distinguishing between normal and tumor tissues and in assessing malignancy in transitional mucosa. In addition, PNA binding can be used to measure cellular maturity in lymphoid tissues, to distinguish a variety of lymphocyte subpopulations in man and experimental animals, and to measure the levels of lymphoid cell populations in many diseases. PNA can be employed in the fractionation of stem cells in mice for use in bone marrow transplantation across histocompatibility barriers.

Texas Red-conjugated PNA has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. Texas Red-conjugated PNA has an excitation at 595-605 nm and an emission at about 615 nm.

Technical Specifications:

Ligand Source: Arachis hypogaea

Molecular Weight: 110 kDa

Carbohydrate Specificity: GalBeta1,3GalNAc (T-antigen)

Inhibiting/Eluting Sugar: Galactose

Carbohydrate for Elution: 0.2M Galactose

Blood Group Specificity: T antigen (M, N)

Conjugate: Texas Red

Form: Liquid

Isoelectric (pI): 5.5-6.5

Storage: -20 C

Application note: Calcium and Magnesium are required for binding.

    APPLICATIONS

    • Biotynylated PNA can be used for the detection of relocalization of Tag antigen in large bowel carcinoma
    • Biotynylated PNA can be used to distinguish between human lymphocyte subsets.
    • Biotynylated PNA can be used as probe in histochemistry and immuno-histochemistry.
    • Biotynylated PNA can be used in human erythrocyte/lymphocyte studies.

    UNIT DEFINITION: One unit will form 1mg purpurogallin in 20 sec from pyrogallol at pH 6.0 at 20 C

    REFERENCES:

    • Application of fluorescence resonance energy transfer techniques to the study of lectin-binding site distribution on Paramecium primaurelia (Protista, Ciliophora) cell surface. Eur J Histochem. (1998) 42: 205-12.
    • Differential infection of polarized epithelial cell lines by sialic acid-dependent and sialic acid-independent rotavirus strains. J Virol. (2001) 75: 11834-50.
    • Effects of soybean extract on morphology and survival of Caco-2, SW620, and HT-29 cells. Nutr Cancer. (2002) 42: 131-40.