Arginase I (human) (rec.) (highly active)

Product Description: Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Arginase is involved in the nitric oxide (NO) pathway and immune cell arginine metabolism. It is fundamentally involved in cancer, inflammation, infections, fibrotic diseases, neurobiology, pregnancy and immune regulation in general.

Alternate Names/Synonyms: EC 3.5.3.1; ARG1; Arginase 1; Type I Arginase; Liver-type Arginase; L-Arginase

Product type: Protein

Source / Host: E. coli

Sequence: Full length human arginase I.

Species Crossreactivity: Human

Biological Activity: 1.6 ±0.2U/µg protein. One unit is defined as the amount of enzyme that converts 1µmol of L-arginine to L-ornithine and urea per min. at 37°C, pH 9.5 (according to protocol from R.T. Schimke, et al.; J. Biol. Chem. 238, 1012 (1963)).

Molecular Weight: 34.7kDa

Purity: >90% (SDS-PAGE)

Concentration: 0.2µg/µl

Formulation: Liquid. In 10mM TRIS-HCl, pH 7.5, containing 1mM beta-mercaptoethanol, 1mM MnCl2 and 50% glycerol.

Handling Advice: Avoid freeze/thaw cycles.

Short Term Storage: -20°C

Long Term Storage: -80°C

Shipping: DRY ICE

Use & Stability: Stable for at least 1 year after receipt when stored at -80°C.

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